目的 建立同时测定小儿清肺丸中葫芦素B、葫芦素E、白花前胡甲素、白花前胡乙素和白花前胡素E 5种活性成分含量的方法，考察⁶⁰Co-γ射线辐照前后有效成分含量的变化。方法 采用Agilent-C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相A为甲醇-乙腈（1∶1），B为0.5%冰醋酸水溶液，梯度洗脱，检测波长分别为230、321 nm，体积流量为1.0 mL/min，柱温25℃。分别选择辐照剂量为2、4、6、8 kGy对小儿清肺丸进行辐照，比较辐照前后小儿清肺丸中5种活性成分含量的变化，并进行成组t检验考察其显著情况。结果 葫芦素B、葫芦素E、白花前胡甲素、白花前胡乙素和白花前胡素E分别在0.049～1.247、0.079～1.973、0.056～1.406、0.028～0.705、0.028～0.693 μg有良好线性关系，平均加样回收率分别为101.2%、99.7%、99.9%、98.9%、100.5%，RSD分别为0.6%、0.5%、1.2%、1.1%、1.2%；经过2、4、6、8 kGy辐照后，小儿清肺丸所含有效成分葫芦素B、葫芦素E、白花前胡甲素、白花前胡乙素和白花前胡素E均有变化，经成组t检验，在超过6 kGy后，葫芦素B辐照前后含量变化显著（P<0.05）。结论 所建立的小儿清肺丸活性成分测定方法回收率高，重复性好，简单实用，可作为小儿清肺丸质控方法。在辐照剂量不超过6 kGy时，各成分变化不显著，可为小儿清肺丸辐照灭菌手段提供参考。

Objective To establish HPLC method for simultaneous determination of five active ingredients of cucurbitacin B, cucurbitacin E, praeruptorin A, praeruptorin B, and praeruptorin E in Infantile Qingfei Pills (IQP), and study the contents changes of the five effective components in IQP before and after ⁶⁰Co-γ ray irradiation. Methods The Agilent-C₁₈ column (250 mm×4.6 mm, 5 μm) was adopted and the detection wavelength was 230 nm and 321 nm with the flow rate of 1.0 mL/min. The mobile phase consisted of A (methanol:acetonitrile) and B (0.5% glacial acetic acid solution) for gradient elution, and column temperature was 25℃. Irradiation does of 2, 4, 6, 8 kGy were selected to irradiate IQP respectively. The contents of five active components in IQP were compared before and after irradiation, and the significant condition was observed by t-test. Results The linear range of cucurbitacin B, cucurbitacin E, praeruptorin A, praeruptorin B, and praeruptorin E were 0.049-1.247, 0.079-1.973, 0.056-1.406, 0.028-0.705, and 0.028-0.693 μg, The average recovery were 101.2%, 99.7%, 99.9%, 98.9%, and 100.5%, and RSD were 0.6%, 0.5%, 1.2%, 1.1%, and 1.2%, respectively. After irradiation of 2, 4, 6, and 8 kGy, the effective components contents of cucurbitacin B, cucurbitacin E, praeruptorin A, praeruptorin B, and praeruptorin E were changed. After t-test in groups, the content change of cucurbitacin B was significantly after irradiation over 6 kGy (P < 0.05). Conclusion The established method has a high recovery rate, good repeatability, which is simple and practical and can be used for quality control of IQP. The changes of each component are not significant with the radiation no more than 6 kGy, which can provide a reference for the sterilization of IQP.