目的 采用HPLC-MS分析国产沉香的特征成分，为国产沉香质量评价体系的建立提供依据。方法 Dionex-Acclaim 120 C₁₈色谱柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.5%甲酸水溶液梯度洗脱；体积流量0.4 mL/min；检测波长254 nm；柱温26℃；ESI源；二级质谱；阳离子模式。利用SPSS软件分别对10批国产沉香进行聚类分析。结果 10批国产沉香经HPLC-MS分析确定14个flidersia类型色酮的共有峰，并与对照品的保留时间和质谱数据进行比对，鉴定了其中7个共有峰。聚类分析将10批样品分为5类，结果表明，生结沉香与熟结沉香的成分差异较大；黄熟香的成分与产地关系明显。结论 建立的对flidersia类型色酮的分析方法可用于沉香样品的质量评价。

Objective HPLC-MS was used to analyze the characteristic constituent of Aquilariae Lignum Resinatum, providing basis for the establishment of quality evaluation system of agarwood. Methods The analysis was carried out on a Dionex-Acclaim 120 C₁₈ column (250 mm×4.6 mm, 5 μm). The mobile phase consisted of acetonitrile and water-acetic acid (100︰0.5) with the flow rate of 0.4 mL/min at 254 nm, and the separation was performed at 26℃. MS experiments were performed using an electrospray ionization tandem mass spectrometry mainly in positive-ion mode. The cluster analysis was performed by SPSS software. Results A total of 14 common peaks of flidersia chromones were characterized by HPLC-MS analysis, and seven of them were identified by comparing their retention times and MS spectra with reference compounds. The 10 batch of samples were divided into five categories by cluster analysis. The result indicated that great difference existed between agarwood produced from living tree and dead tree, and the relationship between constituent and production place of "Huang-Shu" agarwood was obvious. Conclusion The analytical method for common flidersia chromones established in this paper could be used to evaluate the quality of agarwood.

海南省自然科学基金创新研究团队项目（2017CXTD020）；海南省重大科技计划项目（ZDKJ2016004-03）；中国热带农业科学院级创新团队（17CXTD-15）；现代农业产业技术体系建设专项基金资助项目（CARS-21）