目的 建立同时测定枳壳中伞形花内酯、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、川陈皮素、3，5，6，7，8，（3，4）-七甲氧基黄酮、橘红素、橙皮油素的HPLC方法，测定和比较不同周长大小枳壳中的9种化学成分的量。方法 采用Diamonsil C₁₈（250 mm×4.6 mm，5 μm），流动相为甲醇（A）-水（用磷酸调pH至3.0）；梯度洗脱为0～25 min，30%～50% A；25～35 min，50%～70% A；35～40 min，70%～75% A；40～55 min，75%～100% A。体积流量为1.0 mL/min；柱温30℃；检测波长320 nm；进样量20 μL。结果 伞形花内酯、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、川陈皮素、3，5，6，7，8，（3，4）-七甲氧基黄酮、橘红素和橙皮油素分别在0.002 18～0.07μg（r=0.999 8）、0.021 8～0.70μg（r=0.999 9）、0.242 5～7.76μg（r=0.999 8）、0.024 38～0.78μg（r=0.999 4）、0.523 76～16.76μg（r=0.999 3）、0.004 13～0.132μg（r=0.999 6）、0.003 13～0.10μg（r=0.999 3）、0.002 75～0.088μg（r=0.999 6）和0.000 93～0.03μg（r=0.999 3）呈良好的线性关系；其平均回收率（n=6）分别为98.50%、98.80%、99.51%、98.43%、99.64%、99.21%、100.03%、98.75%和101.11%。结论 该法简便，重复性好，可用于枳壳中伞形花内酯、3，5，6，7，8，（3，4）-七甲氧基黄酮等成分的含量测定。

Objective To establish an HPLC method for simultaneous determination of 7-hydroxycoumarin, narirutin, naringin, hesperidin, neohesperidin, nobiletin, 3,5,6,7,8,(3,4)-heptamethoxyflavone, tangeretin, and auraptene from Aurantii Fructus, determinate and compare the content of various chemical components from Aurantii Fructus with different perimeters. Methods The HPLC system consisted of a diamon C₁₈ (250 mm×4.6 mm, 5 μm), the mobile phase was methanol (A)-water (adjusted pH 3.0 with phosphorous acid). The gradient elution conditions:0-25 min, 30%-50% A; 25-35 min, 50%-70% A; 35-40 min, 70%-75% A; 40-55 min, 75%-100% A. The flow rate was 1.0 mL/min and the column temperature was 30℃. The detection wavelength was 320 nm and the injection volume was 20 μL. Results The calibration curves of 7-hydroxycoumarin, narirutin, naringin, hesperidin, neohesperidin, 3,5,6,7,8,(3,4)-heptamethoxyflavone, nobiletin, tangeretin, and auraptene had good linear relationship in the ranges of 0.002 18-0.07 μg (r=0.999 8), 0.021 8-0.70 μg (r=0.999 9), 0.242 5-7.76 μg (r=0.999 8), 0.024 38-0.78 μg (r=0.999 4), 0.523 76-16.76 μg (r=0.999 3), 0.003 13-0.10 μg (r=0.999 3), 0.004 13-0.132 μg (r=0.999 6), 0.002 75-0.088 μg (r=0.999 6), and 0.000 93-0.03 μg (r=0.999 3); And the average recoveries (n=6) of the nine components were 98.50%, 98.80%, 99.51%, 98.43%, 99.64%, 99.21%, 100.03%, 98.75%, and 101.11%, respectively. Conclusion This method can be applied to determinating the components from Aurantii Fructus, including 7-hydroxycoumarin and 3,5,6,7,8,(3,4)-heptamethoxyflavone, etc.

国家自然科学基金项目（81560651）；国家中医药管理局公益性科研专项（201507002-2）；江西省卫生计生委课题（2016A011）