目的 利用SCoT标记对47份栀子种质资源的亲缘关系及遗传多样性进行了研究。方法 采用NTSYS version 2.1软件计算遗传相似性系数，利用UPGMA法构建聚类树状图。结果 从36条引物中筛选出20条多态性丰富的引物对供试材料进行PCR扩增，共获得154条谱带，其中多态性条带120条，多态性条带所占的比率（PPL）达78.14%，Nei-Li相似系数（GS）在0.655 8～0.980 5，平均值为0.784 1；聚类结果显示栀子遗传多样性丰富且亲缘关系复杂。结论 研究表明SCoT标记技术可以很好地应用于栀子种质资源的遗传多样性分析，研究结果可为栀子育种、分类、保存和利用提供可靠的理论依据。

Objective The genetic variation and relationship of 47 pieces of Gardenia jasminoides germplasms were analyzed by SCoT molecular markers. Methods The genetic similarity coefficient was calculated by NTSYS version 2.1 software and the dendrogram was constructed by UPGMA method. Results More than 20 polymorphic primers were screened from 36 primers and PCR amplificated to test materials. The primers generated totally 154 bands among which 120 bands were found to be polymorphic, with an average of 78.14%. The genetic similarity coefficient among the germplasms ranged from 0.655 8 to 0.980 5, and the average content was 0.784 1. The clustering results showed that the genetic diversity of G. jasminoides was rich and the genetic relationship was complex. Conclusion SCoT markers were feasible and effective to analyze the genetic diversity of G. jasminoides germplasm resources. The results provide a reliable theoretical basis for breeding, classification, preservation and utilization of G. jasminoides.

重庆市基础与前沿研究计划项目（cstc2018jcyjAX0553）；重庆市重点产业共性关键技术创新专项资助（cstc2016zdcy-ztzx10003）；国家中药材产业技术体系资助（CARS-21）；重庆市中药材产业技术体系建设项目资助（2017[5]号）；中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”资助（2060302）