目的 通过比对11种虾脊兰属药用植物的rDNA ITS序列，为该属植物的分子鉴定奠定基础。方法 以叶片基因组DNA为材料，利用PCR法克隆虾脊兰属植物的ITS序列，利用Clustal、GenDoc和Mega等软件进行生物信息学分析。结果 11种虾脊兰属植物的ITS全长为652～661 bp，其中ITS1为228～230 bp，ITS2为256～264 bp，5.8 S的长度则完全一致，均为167 bp；ITS1和ITS2分别有44与46个变异位点，其中信息位点数为22和24个，而5.8 S中没有检测到变异位点和信息位点；系统发育分析结果表明，11种虾脊兰属植物之间的遗传距离为0.002～0.081，在发育树上可分为3组，I～Ⅲ组的物种数量分别为5、4和2。结论 11种虾脊兰属植物的ITS序列具有丰富的信息位点，可用于这些药用植物的分子鉴定。

Objective To provide evidences for molecular identification, sequence comparisons of 11 rDNA ITS in Calanthe plants were performed. Methods ITS sequences of Calanthe plants were isolated by using Polymerase Chain Reaction (PCR) method, and then analyzed by software namely Clustal, GenDoc, and Mega. Results The full ITS sequences of 11 Calanthe plants ranged from 652 to 661 bp; ITS1 and ITS2 sequences were 228-230 bp and 256-264 bp in length, respectively, while their 5.8 S sequences were identical with a length of 167 bp. The variable sites in both ITS1 and ITS2 were 44 and 46, respectively. Parsimony information sites of ITS1 and ITS2 were 22 and 24, however, there was no variable and information sites indentified in 5.8 S sequences. Genetic distance among 11 Calanthe species varied from 0.002 to 0.081, and they were divided into three groups in a phylogenetic tree with species numbers of 5, 4, and 2 in I-Ⅲ groups, respectively. Conclusion The ITS sequences of 11 Calanthe plants were rich in information sites, which could be applied for molecular identification.

台州市211人才工程经费（2012年度）