目的 建立防风Saposhnikovia divaricate再生体系。方法 研究不同外植体、温度、培养基、光照条件对诱导防风愈伤组织的影响，采用响应面试验设计，优化愈伤组织诱导及增殖的激素配比。结果 以防风的叶片为外植体，诱导愈伤组织最适培养基为MS＋6-BA 0.90 mg/L＋2，4-D 1.05 mg/L＋KT 0.96 mg/L，25℃恒温12 h/d光照培养；最适增殖培养基为MS＋6-BA 0.56 mg/L＋2，4-D 0.48 mg/L＋KT 0.40 mg/L，继代周期为35 d；诱导丛生芽最适培养基为MS＋NAA 0.6 mg/L＋6-BA 1.0 mg/L；最适生根培养基为MS＋NAA 0.4 mg/L＋6-BA 0.1 mg/L。结论 成功诱导防风愈伤组织并建立稳定高效的植株再生体系。

Objective To establish the regeneration system of Saposhnikovia divaricate (Turcz.) Schisck. Methods The effects of different explants, temperature, medium, and illumination was studied on the induction of S. divaricate. Response surface test design was used to optimize the hormone ratio of callus induction and proliferation. Results The leaves of S. divaricate were used as the experimental materials, the optimal medium for the callus induction was MS + 6-BA 0.90 mg/L + 2,4-D 1.05 mg/L + KT 0.96 mg/L, with temperature of 25℃ and light of 12 h/d. The optimal medium for callus proliferation was MS + 6-BA 0.56 mg/L + 2,4-D 0.48 mg/L + KT 0.40 mg/L, and the subculture cycle was 35 d. The optimal medium for adventitious shoots was MS + NAA 0.6 mg/L + 6-BA 1.0 mg/L. The optimal medium for root growing was MS + NAA 0.4 mg/L + 6-BA 0.1 mg/L. Conclusion S. divaricate callus was successfully induced and a stable and efficient plant regeneration system was established.

甘草等7种中药饮片标准化建设（ZYBZH-Y-JL-25）