目的 研究血人参提取物体内外对乳腺癌4T1细胞的影响。方法 采用MTT法检测血人参不同提取部位体外对4T1细胞的抗肿瘤活性，计算细胞增殖抑制率及半数抑制浓度（IC₅₀）；建立小鼠4T1实体瘤模型，随机分为模型组、血人参不同提取部位（石油醚部位、醋酸乙酯部位、正丁醇部位、水部位、乙醇提取物）组、顺铂组，给药15 d后观察小鼠脏器指数、体质量，计算抑瘤率，进行肿瘤病理切片，观察病理改变。结果 血人参醋酸乙酯部位、正丁醇部位及乙醇提取物对4T1细胞的IC₅₀值分别为228.9、323.4、322.6 μg/mL；血人参石油醚部位组、醋酸乙酯部位组、正丁醇部位组、水部位组、乙醇提取物组及顺铂组对4T1荷瘤小鼠的抑瘤率分别为（55.88±6.68）%、（66.67±14.32）%、（65.71±12.38）%、（53.81±16.17）%、（43.73±25.73）%、（76.85±11.38）%；血人参不同提取部位组中，石油醚部位组具有降低脾指数、升高胸腺指数及白细胞介素（IL-2）水平的作用；从肿瘤体积和抑瘤率结果来看是醋酸乙酯部位最好。HE染色显示石油醚部位组肿瘤细胞明显比模型组少，细胞排列松散，病理性核分裂现象、肿瘤细胞浸润较模型组少，有少量淋巴细胞、巨噬细胞浸润。结论 血人参提取物在体内外对4T1细胞生长具有一定的抑制作用，其体内作用机制可能是增强机体的免疫力，从而达到抑制肿瘤生长的效果。

Objective To study the effects of Indigofera stachyoide extracts on the breast cancer cells (4T1) in vivo and in vitro. Methods MTT method was used to detect the antitumor activity of I. stachyoides extracts in 4T1cells in vitro, the inhibition rate of cell proliferation, and half inhibition concentration (IC₅₀). The established mice model with 4T1 solid tumor were randomly divided into model, extracts of I. stachyoides (petroleum ether phase, ethyl acetate phase, n-butanol phase, water phase, and ethanol extracts) groups, and cisplatin group. After being administered for 15 d, mice body weight and victera index were measured; The observation of tumor pathology and the calculation of tumor inhibition rate were performed. Results IC₅₀ of ethyl acetate phase, n-butanol phase, ethanol extracts of I. stachyoides on 4T1 cells in vitro reached 228.9, 323.4, and 322.6 μg/mL, respectively. The tumor inhibition rates of petroleum ether phase, ethyl acetate phase, n-butanol phase, water phase, and ethanol extract of I. Stachyoides, and cisplatin group on 4T1 mice were (55.88 ±6.68)%, (66.67 ±14.32)%, (65.71 ±12.38)%, (53.81 ±16.17)%, (43.73 ±25.73)%, and (76.85 ±11.38)%, respectively. In the different extraction parts of I. stachyoide, the petroleum ether group had the effects of reducing the spleen index, increasing the thymus index and IL-2 level, and the ethyl acetate part was the best partaccording to tumor volume and the tumor suppressor rate. HE staining showed that the tumor cells in petroleum ether extract group were less than that in the model group, the cell arrangement was loose, the pathological mitosis and tumor cell infiltration were less than those of model group, and there was a small amount of lymphocytes and macrophages infiltration. Conclusion The extracts of I. stachyoides can inhibit the growth of 4T1 tumor cells in vivo and in vitro, and its mechanism may enhance the body immunity, so as to inhibit the tumor growth.

国家工程技术研究中心组建项目（2014FΜ125Q09）；贵州省中药现代化科技产业研究开发专项项目（黔科合中药字［2013］5009）；贵州省普通高等学校工程研究中心建设项目（黔教合KY字［2014］22号）；贵州省高层次创新型人才培养项目（黔科合人才［2015］4030号）；苗医药基本理论的深化、拓展及应用研究（黔科合J重大字［2015］2002-3-5）