目的 建立复黄片全方4味中药的鉴别方法。方法 采用高效液相色谱（HPLC）法定性鉴定复黄片全方君药蒲黄炭中香蒲新苷和异鼠李素-3-O-新橙皮糖苷和臣药槐角中槐角苷，色谱条件为Agilent TC-C₁₈（2）色谱柱（250 mm×4.6 mm，5 μm），柱温30℃，流动相为乙腈-磷酸缓冲盐，梯度洗脱，体积流量为1.0 mL/min，检测波长为254 nm；酸水解-HPLC法定性鉴别臣药地榆炭；采用薄层色谱（TLC）法鉴别佐使药大黄。结果 HPLC法同步定性鉴别了君药蒲黄炭中的香蒲新苷、异鼠李素-3-O-新橙皮糖苷和臣药槐角中的槐角苷，酸水解-HPLC法鉴别了臣药地榆炭，TLC鉴别了佐使药大黄。结论 3种鉴别方法简便易行、专属性强，可鉴别全方4味中药。

Objective To establish the identification method of Typha angustifolia (stir-bake to black), Sophora japonica, Sanguisorba officinalis (stir-bake to black), and Rheum palmatum in Fuhuang tablets. Methods HPLC method were studied to identify typhaneoside and isorhamnetin-3-O-neoheptanoside in T. angustifolia, and sophoricoside in S. japonica. HPLC conditions were as follows:Agilent TC-C₁₈ (2) column (250 mm×4.6 mm, 5 μm) with a gradient elution at a temperature of 30℃; Phosphate buffer and acetonitrile were used as the mobile phase with a flow rate of 1.0 mL/min; The detection wavelength was 254 nm. The acid hydrolysis-HPLC method was explored to identify S. officinalis (stir-bake to black). TLC identification method was performed to identify R. palmatum. Results HPLC method can identify typhaneoside, isorhamnetin-3-O-neoheptanoside, and sophoricoside synchronously. Acid hydrolysis method can identify S. officinalis (stir-bake to black) by HPLC and TLC can identify R. palmatum. Conclusion The three methods are simple and accurate with high sensitivity and good specificity, which can be used to identify all herbal medicines in Fuhuang tablets.

上海市科学技术委员会生物医药领域支撑项目（14401900100）