目的 从桑Morus alba悬浮培养细胞中分离Diels-Alder型加合物。方法 采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、C₁₈柱色谱以及半制备高效液相等多种色谱方法进行分离纯化，结合其理化数据和NMR、MS、ECD等波谱数据鉴定化合物结构。结果 从桑悬浮培养细胞78%乙醇提取物的醋酸乙酯萃取部分中共分离得到8个化合物，分别鉴定为蒙桑素H （1）、川桑素J （2）、蒙桑素F （3）、桑呋喃G （4）、artonin D （5）、桑酮R （6）、川桑素C （7）及桑呋喃E （8）。结论 化合物1~8均为Diels-Alder型加合物，具有中等细胞毒活性，其中1和2为新化合物。

Objective To investigate the Diels-Alder adducts from cell suspension cultures of Morus alba.Methods A variety of column chromatography (CC) including silica gel CC, Sephadex LH-20 CC, C₁₈ CC, and semi-preparative HPLC were used to separate Diels-Alder adducts from cell cultures of M. alba. Their structures were identified by physicochemical properties and various spectroscopic experiments, including MS, NMR, and ECD.Results Eight Diels-Alder adducts were obtained from the ethyl acetate extract of M. alba, and determined as mongolicin H (1), morbilisin J (2), mongolicin F (3), mulberrofuran G (4), artonin D (5), kuwanon R (6), morbilisin C (7), and mulberrofuran E (8).Conclusion Compounds 1-8 are all Diels-Alder adducts and show medium cytotoxic activity, and compounds 1 and 2 are new compounds.

中国医学科学院医学与健康科技创新工程经费资助（CIFMS-2016-I2M-3-012）