目的 研究人体肠道菌群对黄连提取物主要活性成分的代谢及黄连提取物对人体肠道菌群生长的影响。方法 采用UPLC-Q-TOF/MS技术分析肠道菌群对黄连提取物转化后的代谢物；并应用浊度法测定不同质量浓度黄连提取物对4类肠道细菌（乳酸菌、双歧杆菌、肠杆菌和肠球菌）生长的影响。结果 肠道菌群对黄连提取物生物碱类成分主要进行氢化和去甲氧基作用，其中小檗碱和黄连碱被代谢为氢化产物，巴马汀被转化为去甲氧基产物；黄连提取物显著抑制病原菌（肠杆菌、肠球菌）的生长，显著促进益生菌（乳酸菌、双歧杆菌）的生长。结论 肠道菌群对黄连提取物生物碱类成分具有多种代谢作用，且黄连提取物具有改善肠道微生态的功能。

Objective To investigate the interaction between Coptidis Rhizoma extracts and intestinal bacteria.Methods The metabolites of Coptidis Rhizoma extracts by human gut microbiota were identified by UPLC-Q-TOF/MS.Furthermore, the effects of Coptidis Rhizoma extracts on the growth of Bifiobactderia, Lactobacilli, Enterobacter, and Enterococcus were evaluated by the colorimetric method.Results Alkaloids in Coptidis Rhizoma were mainly degraded by reduction and demethoxy, among which berberine and coptisine were converted to reductive products and palmatine was metabolized to a demethoxyl product.Additionally, pathogens such as Enterobacter and Enterococcus were obviously inhibited and probiotics such as Lactobacilli and Bifiobactderia were notably promoted by Coptidis Rhizoma extracts.Conclusion Gut microflora can metabolize alkaloids from Coptidis Rhizoma by multiple ways, and Coptidis Rhizoma extracts can improve the intestinal microecology.

国家自然科学基金资助项目（81673831）