目的 用响应面法优化乙醇浸提蛇莓花色苷的工艺条件并研究其稳定性。方法 在单因素试验基础上，选取乙醇体积分数、液料比、提取时间、提取温度为自变量，花色苷提取率为响应值，采用Box-Behnken设计试验及响应面法分析，优化浸提工艺。研究pH值、温度、有机酸、氧化剂对花色苷稳定性的影响。结果 优化的最佳工艺为乙醇体积分数80%，液料比10：1，提取时间93 min，提取温度36℃，在该最优条件下得到花色苷提取率为1.126%。稳定性实验表明蛇莓花色苷在酸性、低温条件下稳定性好，添加柠檬酸和没食子酸可增强其稳定性，而在氨基乙酸、H₂O₂中稳定性差。结论 响应面法优化所得工艺科学合理、切实可行，可用于蛇莓花色苷的提取；对蛇莓花色苷操作时应注意条件以保持其稳定性。

Objective To optimize extraction technology and evaluate the stability of anthocyanins from Duchesnea indica by response surface methodology. Methods Based on single factor experiment, the ethanol concentration, liquid-material ratio, extraction time, and extraction temperature were selected as independent variables, and the anthocyanin extraction ratio was selected as response value. The Box-Behnken design method and response surface methodology were used to optimize the extraction technology. Additionally, the effects of pH, temperature, organic acid, and oxidizer on the stability of anthocyanins were investigated. Results The optimized extraction technology:ethanol concentration was 80%, liquid-material ratio was 10:1, extraction time was 93 min, and extraction temperature was 36℃. The ratio of the extraction of anthocyanins with the optimal technology was 1.126%. The stability experiments results showed that the anthocyanins should keep in acid and low temperature conditions. Adding citric acid and gallic acid enhanced its stability, but glycine and H₂O₂ decreased stability. Conclusion The extraction technology optimized by response surface methodology was scientific, reasonable, and feasible, and it can be applicable for the extraction of anthocyanins from D. indica. Moreover, the condition should be paid attention to in order to maintain the stability of anthocyanins from D. indica.

河北省自然科学基金项目（H2016209319）；华北理工大学博士科研启动基金项目（25759799）；华北理工大学大学生创新创业训练计划项目（X2017255）；华北理工大学研究生创新项目（2018S49）