目的 建立霍山石斛Dendrobium huoshanense的HPLC指纹图谱，同时测定不同生长年限霍山石斛中丁香酸、芦丁、石斛酚、柚皮素含量，为霍山石斛药材质量的评价提供依据。方法 采用Agilent C₁₈（250 mm×4.6 mm，5 μm）色谱柱，流动相为甲醇-0.1%磷酸水溶液，梯度洗脱，体积流量1.0 mL/min，检测波长240 nm，柱温30℃；将色谱图导入《中药色谱指纹图谱相似度评价系统》（2012.1），对不同年限的霍山石斛样品进行相似度分析；峰面积导入SPSS软件进行聚类分析，从树状图上判断聚类效果。结果 本方法建立的指纹图谱共有13个共有峰，指认了其中4个共有峰，分别为丁香酸、芦丁、石斛酚、柚皮素；不同年限的栽培霍山石斛相似度结果小于0.900，不同年限的仿野生霍山石斛相似度结果大于0.900。结论 HPLC指纹图谱结合化学计量学方法能够评价和鉴别不同年限的霍山石斛，为霍山石斛药材的采收、开发、评价等提供参考。

Objective To establish a HPLC fingerprint method for assessing the quality of Dendrobium huoshanense, in addition to determining concentrations of syringic acid, rutin, dendrophenol and naringenin in this crude drug. Methods Agilent C₁₈ column (250 mm×4.6 mm, 5 μm) was utilized with the mobile phase comprising methanol-0.1% phosphoric acid with the flow rate of 1 mL/min in a gradient elution manner. The detection wavelength was set at 240 nm and the column temperature was 30℃. The resultant chromatograms were imported to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica (2012.1) to obtain retention time and peak area of samples. Similarity for 39 sets of samples were analyzed. The peak area data were processed by SPSS software for cluster analysis and the clustering effect was discussed. Results The line relationship of this way was good (R > 0.999), with high precision regarding instrument used (RSD < 3.00%), the method showed good reproducibility (RSD < 3.00%), standard recovery was between 99.26% and 100.32% (RSD of 0.35%-1.67%). Different growth period and different planting patterns of D. huoshanense were distinct regarding the concentration of four compounds. Conclusion The method is useful to evaluate and discriminate D. huoshanense at different growth period for the purpose of providing scientific reference on harvest, development and evaluation of D. huoshanense.

安徽高校科研创新平台团队项目（皖教秘科[2015]49号）；安徽道地中药材品质提升协同创新中心项目（皖教科[2013]2号）；安徽省教育厅项目（皖教秘科[2014]44号）；安徽省科技重大专项计划项目（16030801104）