目的 探讨消岩汤影响非小细胞肺癌转移的可能作用机制。方法 通过Western blotting检测方法及定量PCR（RT-CR）检测方法检测消岩汤干预下肺癌干细胞中多种炎性蛋白及因子表达的变化，探索消岩汤影响非小细胞肺癌转移的可能作用机制。结果 Western blotting检测方法检测显示实验组较对照组白细胞介素-6（IL-6）、转化生长因子-β（TGF-β）、磷酸化白细胞抑制因子-3（p-Smad3）、基质金属蛋白酶-9（MMP-9）蛋白的表达均有明显的降低（P<0.05），差异具有统计学意义；RT-PCR检测显示实验组较对照组IL-6、TGF-β、TNF-α、MMP-2、MMP-9基因表达均有明显降低（P<0.05），差异具有统计学意义。结论 消岩汤主要通过抑制肺癌炎性微环境中的TGF-β/Smad3信号通路的传导来影响非小细胞肺癌的转移。

Objective To investigate the specific mechanism of Xiaoyan Decoction in the metastasis of non-small cell lung cancer. Methods The detection method of Western blotting method and quantitative PCR Xiaoyan Decoction intervention expression of many inflammatory proteins and cytokines in the lower lung cancer stem Xiaoyan Decoction explore the possible mechanism of effect of non small cell lung cancer metastasis. Results Western blotting detection showed that the expression of IL-6, TGF-β, p-Smad3, and MMP-9 protein in both experimental group and control group decreased significantly (P < 0.05), the difference was statistically significant; RT-PCR showed that the experimental group compared with the control group IL-6, TGF-β, TNF-α, MMP-2, alpha beta MMP-9 gene expression decreased significantly (P < 0.05), the difference was statistically significant. Conclusion Xiaoyan Decoction mainly affects the metastasis of non-small cell lung cancer by inhibiting the transmission of TGF-β/Smad3 signaling pathway in the inflammatory microenvironment of lung cancer.

天津市科技计划项目"扶正解毒祛瘀法方药联合甲磺酸阿帕替尼治疗晚期胃癌放化疗失败患者的临床研究"（17ZXMFSY00190）；天津市科技计划项目"天津市中医内科临床研究中心"（15ZXLCSY00020）；天津市卫生和计划生育委员会"基于LAPTM4B-35-PI3K/AKT信号通路探讨消岩汤在非小细胞肺癌中抗肿瘤作用及其机制研究"（2017003）；天津市卫生和计划生育委员会"从‘同病异治’探讨消岩汤联合阿帕替尼对NSCLC的临床研究"（2017119）；天津市教委课题"消岩汤对肺腺癌A549细胞凋亡生成素基因表达的影响"（20120216）；天津市应用基础与前沿技术研究计划重点项目"消岩汤对肺腺癌A549细胞凋亡生成素基因表达的影响""消岩汤调控肺腺癌A549细胞株SP细胞Wnt信号通路的研究"（14JCZDJC36900）