目的 建立HPLC波长切换联合梯度洗脱法（HPLC-DVD法）同时测定双参龙胶囊（SSLC）中毛蕊异黄酮葡萄糖苷、鲁斯可皂苷元、苦杏仁苷、人参皂苷Rb₁、人参皂苷Re、阿魏酸、西红花苷-I、丹酚酸B、11-羰基-β-乙酰乳香酸和丹参酮Ⅱ_A 10种指标成分的方法。方法 采用HPLC-DVD法，Hypersil ODS C₁₈（150 mm×4.0 mm，3 μm）色谱柱；甲醇-水（8：2，A）-0.1%磷酸水溶液（B）为流动相，梯度洗脱，体积流量0.6 mL/min；毛蕊异黄酮葡萄糖苷的检测波长为260 nm，鲁斯可皂苷元的检测波长为280 nm，苦杏仁苷的检测波长为210 nm，人参皂苷Rb₁和人参皂苷Re的检测波长为203 nm，阿魏酸的检测波长为320 nm，西红花苷I的检测波长为440 nm，丹酚酸B的检测波长为286 nm，11-羰基-β-乙酰乳香酸的检测波长为250 nm，丹参酮Ⅱ_A的检测波长为270 nm；进样量为10 μL。结果 10种指标成分毛蕊异黄酮葡萄糖苷在3.88~69.86 mg/L（r=0.999 2）、鲁斯可皂苷元在22.1~397.8 mg/L（r=0.999 1）、苦杏仁苷在37.43~673.5 mg/L（r=0.999 4）、人参皂苷Rb₁在45.15~812.72 mg/L（r=0.999 6）、人参皂苷Re在4.55~81.95 mg/L（r=0.999 5）、阿魏酸在3.06~55.15 mg/L（r=0.999 4）、西红花苷-I在1.93~34.76 mg/L（r=0.999 5）、丹酚酸B在15.68~282.15 mg/L（r=0.999 6）、11-羰基-β-乙酰乳香酸在11.31~203.58 mg/L（r=0.999 1）、丹参酮Ⅱ_A在1.89~34.16 mg/L（r=0.999 6）质量浓度与峰面积具有较好的线性关系；精密度良好，RSD ≤ 1.27%；重复性良好，RSD ≤ 1.28%；供试品溶液在室温条件下8 h内稳定，RSD ≤ 0.96%；平均加样回收率和相应的RSD分别为99.61%、1.21%，100.11%、0.76%，101.52%、0.62%，101.22%、1.03%，100.83%、1.14%，98.94%、0.53%，101.04%、1.09%，100.05%、1.25%，99.81%、0.68%，101.94%、1.31%。9批次供试品中毛蕊异黄酮葡萄糖苷、鲁斯可皂苷元、苦杏仁苷、人参皂苷Rb₁、人参皂苷Re、阿魏酸、西红花苷-I、丹酚酸B、11-羰基-β-乙酰乳香酸和丹参酮Ⅱ_A量分别为0.142~0.158、0.747~0.764、1.578~1.619、2.163~2.185、0.235~0.251、0.557~0.580、0.105~0.122、0.311~0.328、0.605~0.624、0.062~0.079 mg/粒。结论 建立的HPLC-DVD法同时测定SSLC中的10种成分，方法操作简便、快速、准确，可作为SSLC质量控制的分析方法。

Objective To establish HPLC coupled with wavelength switching and gradient elution method (HPLC-DVD) for simultaneous determination of ten main components (calycosin-7-glucoside, ruscogenin, amygdalin, ginsenoside Rb₁, ginsenoside Re, ferulic acid, crocin I, salvianolic acid B, acetyl-11-keto-β-boswellic acid, and tanshinone Ⅱ_A) in Shuangshenlong Capsule (SSLC). Methods The chromatographic separation was achieved on Hypersil ODS C₁₈ (150 mm×4.0 mm, 3 μm) column with methanol-water (8:2) T (A)-0.1% phosphoric acid solution (B) as mobile phases for gradient elution, at the flow rate of 0.6 mL/min; The detection wavelength was set at 260 nm for α-calycosin-7-glucoside, 280 nm for ruscogenin, 210 nm for amygdalin, 203 nm for ginsenoside Rb₁ and ginsenoside Re, 320 nm for ferulic acid, 440 nm for crocin I, 286 nm for salvianolic acid B, 250 nm for acetyl-11-keto-β-boswellic acid, and 270 nm for tanshinone Ⅱ_A. The volume of sample injection was 10 μL. Results The ten active components were well separated and showed good linearity between mass concentration and peak area, such as calycosin-7-glucoside 3.88-69.86 mg/L (r=0.999 2), ruscogenin 22.1-397.8 mg/L (r=0.999 1), amygdalin 37.43-673.5 mg/L (r=0.999 4), ginsenoside Rb₁ 45.15-812.72 mg/L (r=0.999 6), ginsenoside Re 4.55-81.95 mg/L (r=0.999 5), ferulic acid 3.06-55.15 mg/L (r=0.999 4), crocin I 1.93-34.76 mg/L (r=0.999 5), salvianolic acid B 15.68-282.15 mg/L (r=0.999 6), acetyl-11-keto-β-boswellic acid 11.31-203.58 mg/L (r=0.999 1), and tanshinone Ⅱ_A 1.89-34.16 mg/L (r=0.999 6). The precision was good, and RSD was not more than 1.27%. The repeatability was good, and RSD was not more than 1.28%. The stability was good in 8 h, and RSD was not more than 0.96%. The average recoveries and corresponding RSD values were 99.61% (1.21%), 100.11% (0.76%), 101.52% (0.62%), 101.22% (1.03%), 100.83% (1.14%), 98.94% (0.53%), 101.04% (1.09%), 100.05% (1.25%), 99.81% (0.68%), and 101.94% (1.31%), respectively. The contents of nine batches of calycosin-7-glucoside, ruscogenin, amygdalin, ginsenoside Rb₁, ginsenoside Re, ferulic acid, crocin I, salvianolic acid B, acetyl-11-keto-β-boswellic acid, and tanshinone Ⅱ_A were 0.142-0.158, 0.747-0.764, 1.578-1.619, 2.163-2.185, 0.235-0.251, 0.557-0.580, 0.105-0.122, 0.311-0.328, 0.605-0.624, 0.062-0.079 mg/capsule, respectively. Conclusion HPLC coupled with wavelength switching and gradient elution method has been established for simultaneous determination of ten components in SSLC. The method is simple, quick, accurate, and it can be used for content determination and quality control of SSLC.