目的 研究枸杞Lycium chinense根的化学成分。方法 采用硅胶柱色谱、ODS开放柱色谱、Sephadex LH-20葡聚糖凝胶柱色谱及制备高效液相色谱等方法对化合物进行分离纯化，根据其理化性质及波谱数据鉴定化合物结构。结果 从枸杞根部乙醇提取物的醋酸乙酯部位分离得到12个化合物，分别鉴定为大海米菊酰胺K（1）、grossamide（2）、dihydrogrossamide（3）、大麻酰胺H（4）、1，2-dihydro-6，8-dimethoxy-7-hydroxy-1-（3，4-dihydroxyphenyl）-N¹，N²-bis[2-（4-hydroxyphenyl）ethyl]-2，3-naphthalene dicarboxamide（5）、大麻酰胺D（6）、（1，2-trans）-N³-（4-acetamidobutyl）-1-（3，4-dihydroxyphenyl）-7-hydroxy-N²-（4-hydroxyphenethyl）-6，8-dimethoxy-1，2-dihydronaphthalene-2，3-dicarboxamide（7）、大麻酰胺F（8）、（E）-2-（4，5-dihydroxy-2-{3-[（4-hydroxyphenethyl）amino]-3-oxopropyl}phenyl）-3-（4-hydroxy-3-methoxyphenyl）-N-（4-acetamidobutyl）acrylamide（9）、（E）-2-（4，5-dihydroxy-2-{3-[（4-hydroxyphenethyl）amino]-3-oxopropyl}phenyl）-3-（4-hydroxy-3，5-dimethoxyphenyl）-N-（4-hydroxyphenethyl）acrylamide（10）、（+）-南烛木树脂酚-3α-O-β-D-葡萄糖（11）、（-）-南烛木树脂酚-3α-O-β-D-葡萄糖（12）。结论 化合物3为新天然产物，1、2、4~8为首次从该植物中分离得到。

Objective To study the chemical constituents from the roots of Lycium chinense. Methods The compounds were isolated and purified by silica gel, ODS and Sephadex LH-20 chromatography in addition to preparative-HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral analysis. Results Twelve compounds were isolated from the EtOAc fraction of the root of Lycium chinense and identified as grossamide K (1), grossamide (2), dihydrogrossamide (3), cannabisin H (4), 1,2-dihydro-6,8-dimethoxy-7-hydroxy-1-(3,4-dihydroxyphenyl)-N¹,N²-bis[2-(4-hydroxyphenyl)ethyl]-2,3-naphthalene dicarboxamide (5), cannabisin D (6), (1,2-trans)-N³-(4-acetamidobutyl)-1-(3,4-dihydroxyphenyl)-7-hydroxy-N²-(4-hydroxyphenethyl)-6,8-dimethoxy-1,2-dihydronaphthalene-2,3-dicarboxamide (7), cannabisin F (8), (E)-2-(4,5-dihydroxy-2-{3-[(4-hydroxyphenethyl)amino]-3-oxopropyl} phenyl)-3-(4-hydroxy-3-methoxyphenyl)-N-(4-acetamidobutyl)acrylamide (9), (E)-2-(4,5-dihydroxy-2-{3-[(4-hydroxylphenethyl) amino]-3-oxopropyl}phenyl)-3-(4-hydroxy-3,5-dimethoxyphenyl)-N-(4-hydroxyphenethyl)acrylamide (10), (+)-lyoniresinol-3α-O-β-D-glucopyranoside (11), and (-)-lyoniresinol-3α-O-β-D-glucopyranoside (12). Conclusion Compound 3 is a new natural product. Compounds 1, 2, and 4-8 are isolated from this plant for the first time.

国家自然科学基金面上项目（81573578）