目的 探讨消岩汤对非小细胞肺癌转移的作用机制。方法 通过划痕实验，观察消岩汤对肺腺癌A549细胞的迁移能力的影响；通过侵袭实验，观察消岩汤对肺癌干细胞（SP细胞）侵袭能力的影响。结果 划痕实验中消岩汤组肺腺癌A549细胞在24、48、72 h的迁移距离明显短于各时刻对照组细胞（P<0.05）；侵袭实验中消岩汤干预下的SP细胞穿出Transwell小室的细胞数明显少于对照组（P<0.05）。结论 消岩汤可有效抑制肺腺癌A549细胞的迁移及SP细胞的侵袭。

Objective To study the effect of Xiaoyan Decoction on metastasis of non-small cell lung cancer by experimental study. Methods Through the scratch test, the effect of Xiaoyan Decoction on the migration ability of lung adenocarcinoma A549 cells was observed. The transwell migration assay was used to test the role of Xiaoyan Decoction in lung cancer stem cells migration. Results In the scratch test, the migration distance of lung adenocarcinoma A549 cells in 24, 48, and 72 h was significantly shorter than that of the control group at the time of the scratch test (P < 0.05). In the invasive experiment, the number of lung cancer stem cells passing through the transwell unit in Xiaoyan Decoction group was significantly less than that of the control group (P < 0.05). Conclusion Xiaoyan Decoction can effectively inhibit the invasion ability of lung cancer stem cells, and then affect their metastatic ability.

天津市科技计划项目“扶正解毒祛瘀法方药联合甲磺酸阿帕替尼治疗晚期胃癌放化疗失败患者的临床研究”（17ZXMFSY00190）；天津市科技计划项目“天津市中医内科临床研究中心”（15ZXLCSY00020）；天津市卫生和计划生育委员会“基于LAPTM4B-35-PI3K/AKT信号通路探讨消岩汤在非小细胞肺癌中抗肿瘤作用及其机制研究”（2017003）；天津市卫生和计划生育委员会“从‘同病异治’探讨消岩汤联合阿帕替尼对NSCLC的临床研究”（2017119）；天津市教委课题“消岩汤对肺腺癌A549细胞凋亡生成素基因表达的影响”（20120216）；天津市应用基础与前沿技术研究计划重点项目“消岩汤对肺腺癌A549细胞凋亡生成素基因表达的影响；消岩汤调控肺腺癌A549细胞株SP细胞Wnt信号通路的研究”（14JCZDJC36900）