目的 通过比较炮制前后铁屑中Fe²⁺及辅料诃子中有机酸量的变化，对诃子制铁屑的炮制原理进行初步研究。方法 采用诃子汤煮法与诃子浸泡法制备2种不同的铁屑炮制品，分别采用紫外分光光度（UV）法和HPLC法测定炮制前后铁屑中Fe²⁺及诃子中5种有机酸量的变化。结果 铁屑生品含Fe²⁺ 0.000 3%，诃子汤煮制铁屑和诃子浸泡制铁屑中Fe²⁺分别升高到3.07%和1.02%；诃子生品含没食子酸1.67%、柯里拉京2.23%、诃黎勒酸9.33%、鞣花酸1.18%、诃子酸24.70%，炮制后有机酸量明显降低，诃子汤煮制铁屑和诃子浸泡制铁屑中没食子酸量分别为1.24%、1.51%，其余4种有机酸量均降为0%。结论 采用诃子炮制铁屑，能够使铁屑生品中的Fe³⁺转化为人体易于吸收的Fe²⁺，铁屑炮制品中剩余的有机酸能够起到抗氧化剂的作用，防止Fe²⁺被氧化为Fe³⁺，延长铁屑炮制品的保存期。

Objective To investigate the principle of scrap iron processing by Terminalia chebula by comparation of the content changes of the Fe²⁺in scrap iron and the organic acid in ingredients of T. chebula before and after processing. Methods Scrap iron was processed by two methods of decocting and immersion, respectively. Spectrophotometric method and HPLC were used to analyze the contents of Fe²⁺ and organic acid before and after processing. Results The content of Fe²⁺ increased from 0.000 3% to 3.07% and 1.02% after processed by the above two methods, respectively. The content of organic acid decreased significantly after processing (1.67% to 1.24% and 1.51% for gallic acid, 2.23% to 0% for corilagin, 9.33% to 0% for chebulagic acid, 1.18% to 0% for ellagic acid, 24.70% to 0% for chebulinic acid). Conclusion The Fe³⁺ in crude scrap iron can be reduced into Fe²⁺ absorbed easily by human in scrap iron processing by T. chebula. Organic acids in the processed products can prevent Fe²⁺ from being oxidized to Fe³⁺, thereby prolonging the retention period.

成都中医药大学实验技术项目（Y1608，SYYB20170022）