目的 建立天麻药材中天麻素、对羟基苯甲醇、巴利森苷A、巴利森苷B、巴利森苷C、巴利森苷E 6种天麻素类成分的一测多评测定方法。方法 采用Agilent Zorbax SB-C₁₈（250 mm×4.6 mm，5μm）色谱柱，以乙腈（A）-0.05%磷酸水溶液（B）为流动相，梯度洗脱，0~5 min，2% A；5~20 min，2%~30% A，20~25 min，30%~2% A，检测波长220 nm，体积流量1.0 mL/min，柱温25℃。以天麻素为内参物，分别建立对羟基苯甲醇、巴利森苷A、巴利森苷B、巴利森苷C、巴利森苷E的相对校正因子，利用该相对校正因子计算其他5种成分的量，同时利用外标法测定天麻素、对羟基苯甲醇、巴利森苷A、巴利森苷B、巴利森苷C、巴利森苷E的量，比较2种测定方法的差异，验证一测多评法的可行性和准确性。结果 在线性范围内，天麻素与对羟基苯甲醇、巴利森苷A、巴利森苷B、巴利森苷C、巴利森苷E的相对校正因子分别为0.663、1.278、1.435、1.389、1.591，在不同实验条件下相对校正因子重现性良好，6种天麻素类成分的计算值与实测值无显著性差异。结论 一测多评法测定天麻药材中6种天麻素类成分准确且可行，可用于天麻药材的质量控制。

Objective To establish a quantitative analysis of multi-components by single marker (QAMS) for determining contents of six gastrodin compositions in Gastrodia elata, gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C and parishin E.Methods Separation was carried out on Agilent Zorbax SB-C₁₈ Column (250 mm×4.6 mm, 5 μm) by using acetonitrile (A)-0.05% Phosphoric acid aqueous solution (B) as the mobile phase, and a linear gradient elution was employed (0-5 min, 2% A, 5-20 min, 2%-30% A, 20-25 min, 30%-2% A) with detection wavelength at 220 nm, flow velocity at 1.0 mL/minand column temperature at 25℃. Five relative correction factors (RCFs) of the five compositions using gastrodin as the internal standard were applied to determine their contents in all samples It was established to determine the contents of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, parishin E by the external standard method (ESM) at the same time. The feasibility and accuracy of QAMS method were verified by comparing the content results determined by ESM and QAMS.Results Within the linear range, the RCFs of gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, parishin E were 0.663, 1.278, 1.435 and 1.591, respectively, with good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method.Conclusion The QAMS method is feasible and accurate for the determination of the six chemical compositions, and which can be used for quality control of G. elata.

山东省中药炮制技术传承基地建设项目；山东省中医药科技发展计划项目（2017-084）；“重大新药创制”科技重大专项（2012ZX09102201-004）