目的 探讨薄荷酚类部位体外抗流感病毒作用，并建立薄荷酚类部位HPLC特征图谱，用于薄荷酚类部位的质量评价。方法 MTT法检测先加药物再接种病毒、药物与病毒同时作用、先接种病毒再加药物3种加药方式下薄荷酚类部位抗PR8病毒感染MDCK细胞的作用；HPLC法测定12批市售薄荷药材酚类部位样品，建立特征图谱；并采用"中药指纹图谱相似度评价系统（2.0版）"进行相似度分析。结果 薄荷酚类部位质量浓度为0.31~10μg/mL在3种加药方式下均有不同程度抗PR8病毒活性，尤其在先加药物再接种病毒、先接种病毒再加药物2种加药方式下，6个稀释质量浓度的薄荷酚类部位均具有明显的抗PR8病毒活性，抑制率分别为14.17%~41.31%、45.64%~87.48%；选取了11个共有特征峰，12批样品相似度在0.9以上，说明各批薄荷酚类部位整体相似度较高。结论 薄荷酚类部位具有较好的体外抗病毒效果；建立的薄荷酚类部位HPLC特征图谱方法简便、稳定、重复性好，能够反映酚类部位的整体面貌，为薄荷酚类部位的质量控制和药效稳定提供参考。

Objective To explore the anti-influenza virus effect of phenolic fraction of Mentha haplocalyx in vitro, and to establish the HPLC characteristic spectrum of phenolic fraction of M. haplocalyx for its quality evaluation.Methods MTT method was used to detect the inhibitory effect of phenolic fraction of M. haplocalyx on influenza virus PR8 infecting MDCK cells in three ways, including adding drug firstly, adding drug and virus simultaneously and adding virus firstly. The characteristic spectrum of phenolic fraction samples of 12 batches of M. haplocalyx was established by HPLC method. The similarity of which was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica (2.0 version).Results The phenolic fraction at 0.31-10 μg/mL showed antivirus PR8 activities in different degrees under three adding drug ways. Phenolic fraction of M. haplocalyx at six dilution concentrations displayed obvious antivirus PR8 effect under two adding drug ways including adding drug firstly and adding virus firstly, and virus inhibition rates of which were 14.17%-41.31% and 45.64%-87.48%, respectively. Total of 11 peaks were chosen as the common characteristic peaks of spectrum with the similarity degrees more than 0.9 of 12 batches of samples, which illustrated that different batches of phenolic fraction of M. haplocalyx were of high similarity.Conclusion Phenolic fraction of M. haplocalyx had better antiviral effect in vitro. The characteristic spectrum method established in this paper was simple, stable and reproducible, which could reflect the whole profile of phenolic fraction of M. haplocalyx and provide reference for quality control and efficacy stability of phenolic fraction of M. haplocalyx.

国家自然科学基金资助项目（81173520）