目的 基于G1-熵权法和正交设计优选黄芪百合颗粒的提取纯化工艺，为其工业化生产提供参考。方法 采用基于G1-熵权法的主客观组合赋权法和L₉（3⁴）正交设计，以提取次数、提取时间和加水量为考察因素，毛蕊异黄酮葡萄糖苷、橙皮苷、粗多糖、醇浸出物提取量及出膏率为评价指标，优选黄芪百合颗粒的最佳水提工艺；以多通道管状陶瓷超滤膜的膜孔径、操作压强和滤过温度为考察因素，毛蕊异黄酮葡萄糖苷、橙皮苷、粗多糖、醇浸出物保留率及除杂率为评价指标，优选其最佳超滤纯化工艺。结果 黄芪百合颗粒的最佳水提工艺为用总量20倍的水加热回流提取2次，每次提取75 min；最佳超滤工艺为膜孔径50 nm，操作压强0.10 MPa，滤过温度45℃。在此条件下，3批验证试验数据组间均无明显差异。结论 优选的提取、纯化工艺稳定可行，可为黄芪百合颗粒的工业化生产提供实验依据。

Objective To determine the optimum extraction and purification technology of Huangqi Baihe Granules (HBG) by using orthogonal design and combination empowerment based on G1-entropy method, so as to provide a reference for the industrial production.Methods With the ethanol extraction amount and paste-forming rate of calycosin7-O-β-D-glucopyranoside, hesperidin, crude polysaccharide as evaluation index, and with the extraction times, extraction time and the water adding amount as investigate factors, then the combination empowerment method based on G1-entropy method and orthogonal design were used to optimize the extraction technology of HBG. The same combined methods were used to optimize the purification technology of HBG with retention rate and removal rate of impurity of ethanol extract of calycosin7-O-β-D-glucopyranoside, hesperidin and crude polysaccharide, as evaluation index, and with the membrane pore diameter, operating pressure and filtration temperature of multi-channel tubular ceramic ultrafiltration membrane as investigate factors.Results The test results showed that the optimum extraction technology was as follows:Extracted 2 times with 20 times the amount of water and each for 75 min. The optimum ultrafiltration technology was as follows:Multi-channel tubular inorganic ceramic membrane at 50 nm, operating pressure at 0.10 MPa, filtration temperature at 45℃. Under such condition, there was no significant difference between verification groups of three batches.Conclusion The optimized extraction and purification process is stable and feasible by verification, which can provide experimental basis for industrial production of HBG.

甘肃省科技支撑计划项目（120FKCA169）；陇原青年创新人才扶持计划（2014-93-72）；甘肃省中药新产品创制工程实验室开放基金项目（ZYFYZH-KJ-2015-001）