目的 探讨清燥救肺汤（QJD）及其拆方对肺炎支原体（MP）感染小鼠肺组织Bax、Bcl-2、Caspase-3凋亡蛋白表达的影响。方法 将Balb/c小鼠随机分成对照组、模型组、QJD组、QJD拆方I组、QJD拆方Ⅱ组、阿奇霉素组。除对照组小鼠外，其余5组采用滴鼻法对实验Balb/c小鼠进行MP感染。透射电镜观察肺组织超微结构改变和细胞凋亡，运用免疫组织化学SP法和Western blotting法检测肺组织Bcl-2、Bax、Caspase-3蛋白的表达，并采用qRT-PCR法检测Caspase-3 mRNA的表达。结果 MP感染后，小鼠肺组织出现炎症改变，肺泡壁增厚、肺泡上皮细胞破坏、细胞浸润，并发现凋亡的特征改变。MP感染后的小鼠肺组织Bcl-2、Bax、Caspase-3蛋白的表达明显升高，但是Bcl-2/Bax的值明显下降；与模型组相比，QJD组、QJD拆方I组及阿奇霉素组的Bcl-2的表达升高，Bcl-2/Bax的值也明显升高，Bax、Caspase-3的表达下降；QJD拆方Ⅱ组与模型组比较各蛋白表达差异不明显。Caspase-3 mRNA检测结果显示，QJD组、QJD拆方I组及阿奇霉素组表达较模型组降低，QJD拆方Ⅱ组的改变不明显。结论 QJD能够抑制MP感染诱导的细胞凋亡，Bax、Bcl-2可能为其效应靶点之一，其中QJD拆方I起主要作用。

Objective To explore the effect of Qingzao Jiufei Decoction (QJD) and its decomposing agent on the expression of Bax, Bcl-2, and Caspase-3 apoptosis protein in MP infection, in order to determine the effect target of QJD and its decomposing agent. Methods A total of 120 balb/c mice were randomly divided into normal group (group A), model group (group B), QJD group (group C), QJD group I decomposition agent (Group D), QJD group Ⅱ decomposition agent (Group E), and azithromycin group (Group F), 20 rats in each group. Except the normal group, the other five groups were infected with MP by using the nose drop method. The ultrastructure and apoptosis of lung tissue were observed by transmission electron microscope. The expression of Bcl-2, Bax and Caspase-3 protein in lung tissue was detected by Immunohistochemical SP and Western blot method. The expression of Caspase-3 m-RNA was detected by qPCR method. Results After MP infection, inflammation changes can be observed in the lung tissue of mice, thickening of the alveolar wall, destruction of alveolar epithelial cells, cell infiltration, and changes in the characteristics of apoptosis. The expression of Bcl-2, Bax, Caspase-3 protein in the lung tissue of mice infected with MP was significantly increased, but the ratio of Bcl-2/Bax decreased significantly. Compared with the group B, the expression of Bcl-2 in group C, D, and F increased, the ratio of Bcl-2/Bax increased significantly, and the expression of Bax and Caspase-3 decreased. The difference of expression between group E and group B was not obvious. The results of Caspase3 mRNA detection showed that the expression of group C, group D and group F was lower than that of model group, and the change of group E was not obvious. Conclusion QJD can inhibit the cell apoptosis induced by MP infection, and Bax, Bcl-2 were one of its effect target, in which I decomposition agent plays a major role.

国家自然科学基金面上项目（81373687）；辽宁省科技厅项目（2014020044）