目的 优选龙钻通痹方（LTR）总生物碱的最佳纯化工艺。方法 采用5种型号的大孔树脂对LTR总生物碱进行吸附与解吸，以总生物碱的吸附量、吸附率和解吸率为指标，筛选出较佳大孔树脂；然后通过单因素考察和星点设计-效应面法优选LTR总生物碱的最佳纯化工艺参数。结果 HPD100树脂纯化龙钻总生物碱的最佳工艺条件为pH值1.7的生药质量浓度0.17 g/mL，以1 mL/min体积流量上样，9倍柱体积80%乙醇以1 mL/min洗脱。在该条件下，LTR总生物碱提取物中总生物碱的质量分数达到了22.23%，产品得率为173.27 mg/g。结论 HPD100型大孔树脂能较好地用于分离纯化LTR总生物碱，为进一步的药效学研究提供依据。

Objective To optimize the optimal purification technology for total alkaloids from Longzuan Tongbi Recipe (LTR). Methods Five types of macroporous resins were used to adsorb and desorb the total alkaloids in LTR, and the adsorption capacities, adsorption ratios and desorption ratios of total alkaloids were regarded as the indexes to screen out the suitable resin. The purification technology for total alkaloids was optimized from LTR by single factor investigation and central composite design and response surface method. Results The best purification technology of total alkaloid from LTR were determined as follows:the concentration of sample solution of 0.17 g/mL (crude drug) with pH 1.7, sample flow rate at 1 mL/min, washing impurity with 9-column volume of 80% ethanol at a flow rate of 1 mL/min. The mass scores of total alkaloids was 22.23% and the yield of that was 173.27 mg/g. Conclusion HPD100 resin has good purification effect on total alkaloids from LTR, which could provide reference for the further study of pharmacodynamics.

广西高校壮医方药基础与应用研究重点实验室课题（桂教科研[2014]6号zyfy201503，桂教科研[2016]6号zyfy201608）；南宁市科技局项目（20163353）；广西壮族自治区科技厅项目（桂科AB16380186）