目的 研究定心藤属植物定心藤Mappianthus iodoides枝叶中的化学成分。方法 综合运用正相硅胶柱色谱、反相硅胶柱色谱和Sephadex LH-20凝胶柱色谱以及制备型高效液相色谱等方法进行系统分离，根据理化性质及波谱学数据鉴定化合物的化学结构。结果 从定心藤枝叶的90%乙醇提取物中分离得到了16个化合物，分别鉴定为9-hydroxy-4，6-megastigmadien-3-one（1）、9-hydroxy-4，7-megastigmadien-3-one（2）、blumenol A（3）、9，10-dihydroxy-4，7-megastigmadien-3-one（4）、5，12-epoxy-9-hydroxy-7-megastigmen-3-one（5）、5，12-epoxy-6，9-hydroxy-7-megastigmen-3-one（6）、黑麦草内酯（7）、落叶松树脂醇（8）、异落叶松树脂醇（9）、5'-甲氧基落叶松树脂醇（10）、橄榄脂素（11）、去氢双松柏醇（12）、chushizisin I（13）、3，3-didemethoxyverrucosin（14）、4-epi-larreatricin（15）和glycerol monolinoleate（16）。其中化合物1 ~7 为降碳倍半萜类化合物，化合物8 ~15 为木脂素类化合物，化合物16 为脂肪酸类化合物。结论 所有化合物均为首次从定心藤属植物中分离得到。

Objective To study the chemical constituents of the stems and leaves of Mappianthus iodoides. Methods The chemical constituents of M. iodoides were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were identified by physicochemical properties, spectroscopic analysis, as well as comparisons with the data reported in literature. Results Sixteen compounds were isolated from the 90% ethanol extract of the stems and leaves of M. iodoides, which were identified as 9-hydroxy-4,6-megastigmadien-3-one (1), 9-hydroxy-4,7-megastigmadien-3-one (2), blumenol A (3), 9,10-dihydroxy-4,7-megastigmadien-3-one (4), 5,12-epoxy-9-hydroxy-7-megastigmen-3-one (5), 5,12-epoxy-6,9-hydroxy-7-megastigmen-3-one (6), loliolide (7), lariciresinol (8), isolariciresinol (9), 5'-methoxylariciresinol (10), olivil (11), dehydrodiconiferyl alcohol (12), chushizisin I (13), 3,3-didemethoxyverrucosin (14), 4-epi-larreatricin (15), and glycerol monolinoleate (16). Conclusion All compounds are isolated from the genus Mappianthus for the first time.

国家自然科学基金资助项目（31660097，21662011，21302181）；海南省自然科学基金项目（20162024，20162021）；教育部创新团队项目（IRT-16R19）；广西中药质量标准研究重点实验室开放课题（桂中重开201504）