目的 通过比较淫羊藿Epimedium brevicormon不同洗脱部位及其单体化合物对Aβ_25-35诱导的SH-SY5Y神经细胞损伤的保护作用，筛选淫羊藿活性部位和活性成分。方法 淫羊藿提取物经大孔树脂柱、硅胶柱、Sephadex LH-20及制备液相的分离纯化，得到不同洗脱部位和单体化合物。运用Aβ_25-35体外诱导的神经细胞损伤模型，采用Hoechst33342和PI双染法，对淫羊藿的不同洗脱部位和成分进行活性筛选。结果 与模型组比较，除淫羊藿95%乙醇部位外，所有洗脱部位及其中的单体化合物山柰酚-3-O-[α-L-鼠李糖基（1→6）-β-D-半乳糖苷]-7-O-α-L-鼠李糖苷、淫羊藿苷、朝藿定A、朝藿定B均对Aβ_25-35所致SH-SY5Y细胞损伤有显著保护作用。结论 淫羊藿对Aβ_25-35诱导的SH-SY5Y细胞损伤具有保护作用，其二氯甲烷-甲醇（5∶1）部位作用最强，其次是30%乙醇部位、50%乙醇部位。

Objective To compare the neuroprotective effect of different elution fractions and compounds of Epimedium brevicormon on SH-SY5Y cells injury induced by Aβ_25-35 so as to screen pharmacologically fraction and compounds. Methods The isolation and purification of extract from E. brevicormon were performed on D101 macroporous resin column chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, and preparative liquid chromatography. Hoechst 33342/PI double staining method was used to screen neuroprotective fractions and components. Results Compared to model group, all fractions and kaempferol-3-O-[α-L-rhamnopyranosyl (1→6)-β-D-galactoside]-7-O-α-L-rhamnopyranoside, icariin, epimedin A, and epimedin B from E. brevicormon could significantly increase the cells viability, except of 95% ethanol fraction. Conclusion E. brevicormon show some protective effect against Aβ_25-35 induced SH-SY5Y cells injury. The CH₂CI₂-MeOH (5:1) extract from E. brevicormon has the most pharmacologically activity, followed by 30% ethanol and 50% ethanol extracts from E. brevicormon.

科技部重大新药创制项目：现代中药创新集群与数字制药技术平台（2013ZX09402203）