目的 研究藏药印度獐牙菜Swertia chirayita的化学成分。方法 采用硅胶、MCI、Sephadex LH-20等色谱方法进行分离纯化，依据理化性质、波谱数据分析进行结构鉴定。结果 从印度獐牙菜85%乙醇提取物的醋酸乙酯萃取部位分离并鉴定了12个化合物，包括2个（口山）酮、4个三萜、3个裂环烯醚萜和3个其他类化合物，分别鉴定为1，5，8-三羟基-3-甲氧基（口山）酮（1）、1，3，5，8-四羟基（口山）酮（2）、齐墩果酸（3）、4-epi-hederagenin（4）、2-epi-corosolic acid（5）、熊果酸（6）、当药苦酯苷（7）、swerimilegenin I（8）、红白金花内酯（9）、儿茶酚（10）、丁香酸（11）、4-羟基-3-甲氧基苯甲酸（12）。结论 化合物4、5、11 首次从獐牙菜属植物中分离得到，化合物8、9 首次从印度獐牙菜中分离得到。

Objective To investigate the chemical constituents of Swertia chirayita. Methods Column chromatography, such as silica gel, MCI, Sephadex LH-20 were used to isolate and purify the compounds. Physicochemical properties and spectroscopic methods were used to elucidate their structures. Results Twelve compounds, including 2 xanthones, 4 triterpenoids, 3 secoiridoids, and 3 other compounds, the chemical constituents were isolated from the ethyl acetate fraction from 85% ethanol extract of S. chirayita, and identified as bellidifolin (1), norbellidifolin (2), oleanolic acid (3), 4-epi-hederagenin (4), 2-epi-corosolic acid (5), ursolic acid (6), amarogentin (7), swerimilegenin I (8), erythrocentaurin (9), pyrocatechol (10), syringic acid (11), and 4-hydroxy-3-methoxybenzoic acid (12). Conclusion Compounds 4, 5, and 11 are isolated from genus Swertia for the first time, compounds 8 and 9 are found from S. chirayita for the first time.

国家自然科学基金资助项目（31500278）；江苏省药学会奥赛康临床药学研究基金（210303）；南京医科大学药学院大学生创新训练计划项目（2017YXDC02）