目的 克隆多穗柯的查耳酮异构酶（chalcone isomerase，CHI）基因，并了解其表达情况。方法 根据转录组测序结果，利用PCR扩增技术获取多穗柯CHI基因的cDNA全长，并对其进行生物信息学分析，采用qRT-PCR法检测CHI基因在多穗柯不同器官的表达量。结果 多穗柯CHI基因的cDNA全长为772 bp，开放阅读框长为696 bp，编码231个氨基酸的蛋白。该蛋白不存在跨膜区域，定位于细胞质中。多穗柯CHI基因在不同部位均有表达，叶片的表达量最高，是根部最低量的9.75倍。结论 首次克隆获得多穗柯的CHI基因，明确该基因属于CHI Ⅱ型。且多穗柯CHI基因在各器官中的表达量具有显著差异。

Objective To understand the chalcone isomerase (CHI) gene expression by cloning it in Lithocarpus polystachyus. Methods A full-length cDNA of CHI gene from Lithocarpus polystachyus (Lpr-CHI) was obtained by PCR cloning technique according transcriptomics sequences infromation, which bioinformatics analysis was carried out. The expression of CHI gene in different organs of Lithocarpus polystachyus was detected by qRT-PCR. Results Lpr-CHI was 772 bp in full length with an open reading frame (ORF) of 696 bp, which encoded a protein with 231 amino acids. The protein did not contain a transmembrane domain and is localized in the cytoplasm. Lpr-CHI gene expression was found in different parts, and reached the highest in leaf, which was 9.75 times of the least gene expression in root. Conclusion Lpr-CHI was obtained for the first time, and it was clear that the gene belongs to CHI type Ⅱ. And the expression of Lpr-CHI in each organ was significantly different.

国家自然科学基金资助项目（31760221）；华北理工大学培育基金资助项目（SP201508）