目的 采用高效液相色谱-二极管阵列检测器-蒸发光检测器（HPLC-DAD-ELSD）联用技术，建立苦丁茶冬青叶的指纹图谱。方法 岛津C₁₈色谱柱（250 mm×4.6 mm，5μm），以乙腈-水为流动相进行梯度洗脱，体积流量1.0 mL/min，检测波长210 nm，柱温35℃。结果 通过10批苦丁茶冬青药材的指纹图谱，建立了共有模式，其中HPLC-DAD指纹图谱共有峰18个，相似度为0.911～0.970，HPLC-ELSD指纹图谱共有峰11个，相似度为0.914～0.962。结论 方法精密度、重现性、稳定性好，为客观评价苦丁茶冬青药材的质量提供了科学依据。

Objective In this paper, HPLC-DAD-ELSD technique was used to establish the chromatography fingerprint of Ilex kudingcha. Methods Shimadzu C₁₈ column (250 mm×4.6 mm, 5 μm) was used. Acetonitrile-water as the mobile phase; The flow speed was 1.0 mL/min. The detection wavelength was 210 nm and the column temperature was 35℃. Results The chromatography fingerprint of Ilex kudingcha from 10 different origins was established. In the chromatography fingerprint with HPLC-DAD of Ilex kudingcha, 18 common peaks were damarcated and the similarities of Ilex kudingcha were between 0.911—0.970, and the chromatography fingerprint with HPLC-ELSD of Ilex kudingcha, 11 common peaks were damarcated and the similarities of Ilex kudingcha were between 0.914—0.962. Chlorogenic acid, kaempferol-3-O-β-D-rhamnoside, isorhamnetin-3-O-β-D-rhamnoside, kudinoside C, kudinoside A, kudinoside E, kudinoside D were confired by LC-MS and binding control. Conclusion The method of precision, reproducibility, stability is accurate, which can be used as the evaluation of the quality of Ilex kudingcha.

吉林省科技发展计划（20150311037YY）；吉林省卫生计生科研计划项目（2014z206）；吉林省中医药科技项目（2014-ZD28）