目的 建立大黄Rheum palmatum13个有效成分的HPLC测定方法以及不同产地大黄的质量评价模式。方法 采用ZORBAX C₁₈色谱柱（250 mm×4.6 mm，5μm），以甲醇-0.1%磷酸水为流动相，梯度洗脱，体积流量1.0 mL/min，254 nm波长下对没食子酸、表儿茶素、儿茶素、芦荟大黄素苷、大黄酸苷、大黄素苷、大黄酚苷、大黄素甲醚苷、芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚进行测定，对结果进行主成分分析。结果 建立了大黄13个成分的线性回归方程，精密度以及重复性的RSD值在5.00%以内，加样回收率在95.54%～103.19%，样品24 h内稳定；27批样品大黄的量存在较大差异，主成分分析得到5个特征根，主成分综合模型值在0.335～1.905，四川绵阳大黄值最高。结论 建立了大黄13个主要成分的测定方法。主成分分析为大黄药材质量评价提供一种手段，主成分综合模型值可作为大黄质量的评价依据。

Objective To establish an HPLC method for the simultaneous determination of thirteen effective components in Rhei Radix et Rhizoma and to assess the quality from different producing areas. Methods The analysis was performed on ZORBAX C₁₈ chromatographic column with ographic mobile phase consisted of methanol-0.1% phosphoric acidwater in a gradient mode. The flow rate was 1.0 mL/min. Gallic acid, catechin, epicatechin, aloe-emodin-8-O-glucopyranoside, rhein-8-O-glucopyranoside, emodin-8-O-glucopyranoside, physcionin-8-O-glucopyranoside, chrysophanol-8-O-glucopyranoside, aloe-emodin, rhein, emodin, chrysophanol and physcionemod were determined at 254 nm, and the results were analyzed by principal component analysis (PCA). Results The linear regression equation of 13 components of Rhei Radix et Rhizoma was established within a certain concentration range. The precision and reproducibility were less than 5.00%, the recovery of the method was 95.54%—103.19%, and the sample was stable within 24 h. The contents of Rhei Radix et Rhizoma in 27 batches were differences. Five principal components were obtained by PCA, the principal component integrated model was between 0.335 and 1.905 and the value of Rhei Radix et Rhizoma in Mianyang of Sichuan Province was the highest. Conclusion A method for the determination of thirteen main components of Rhei Radix et Rhizoma was established. PCA was used to evaluate the quality of Rhei Radix et Rhizoma and the main component model value can be used as the evaluation index of Rhei Radix et Rhizoma.

国家重点基础研究发展计划（2012CB723502）；四川省青年科技基金项目（2013JQ0018）；四川省科技厅省青年科技创新研究团队专项计划项目（2015TD0028）；四川省科技厅省青年科技创新研究团队专项计划项目——中药药理四川省青年科技创新研究团队（2014TD0007）