目的 初步探索接种不同外源性丛枝菌根（AM）真菌对滇重楼Paris polyphylla var.yunnanensis幼苗内源激素水平变化规律。方法 以灭菌土壤为生长基质，于室温盆栽条件下对滇重楼新鲜种子与28种AM真菌共培养后采样，采用HPLC测定和比较分析各处理组滇重楼根茎和须根内源激素玉米素核苷（ZR）、赤霉素（GA）、吲哚乙酸（IAA）和脱落酸（ABA）的量。结果 接种外源性AM真菌能提高滇重楼幼苗菌根侵染率和成苗率，AM真菌对滇重楼幼苗根茎和须根中ZR、GA、IAA、ABA 4种激素量的变化表现出不同的规律，但具有偏好性。与对照组相比，整体上接种AM真菌处理组根茎中ZR、GA量呈现增长的趋势，IAA量变化规律不明显，而ABA量呈现减少的趋势，接种AM真菌处理组根茎中ABA/ZR、ABA/GA、ABA/IAA值呈现减少的趋势，但须根与根茎的变化趋势相反。结论 AM真菌能促进滇重楼幼苗的存活率，且不同AM真菌菌株影响滇重楼内源激素量。

Objective In order to study the dynamic variation of endogenous hormones in Paris polyphylla var. yunnanensis inoculated by different foreign arbuscular mycorrhizal fungi species. Methods With sterilized soil as growth substrate, the fresh seeds of P. polyphylla var. yunnanensis were co-cultured with 28 arbuscular mycorrhizal (AM) fungi species under the condition of pot culture at room temperature. The rhizomes and fibrils of P. polyphylla var. yunnanensis were collected, and the contents of endogenous hormone zygotic nucleotides (ZR), gibberellin (GA), indole acetic acid (IAA) and abscisic acid (ABA) were determined by HPLC, then content and proportion of endogenous hormones were analyzed, respectively. Results The results showed that it could increase the mycorrhizal colonization rate and seedling rate of P. polyphylla var. yunnanensis inoculated by different foreign AM fungi species. The content change of ZR, GA, IAA and ABA appears different in the rhizomes and seedling fibrils of P. polyphylla var. yunnanensis after the inoculation of AM fungi species, but it has preferences. Compared with the control group (CK), the content of ZR and GA rose obviously in the rhizomes and seedling fibrils of P. polyphylla var. yunnanensis after the inoculation of AM fungi species, the content of ABA reduced and the content of IAA did not obvious change. The ABA/ZR proportion, ABA/GA proportion and ABA/IAA proportion reduced obviously in the rhizomes of P. polyphylla var. yunnanensis after the inoculation of AM fungi species but there was different change in seedling fibrils of P. polyphylla var. yunnanensis. Conclusion In conclusion, it is inferred that the AM fungi can promote the survival rate of the P. polyphylla var. yunnanensis seedlings, and the different AM fungi strains affect the content of endogenous hormones of P. polyphylla var. yunnanensis.

国家自然科学基金项目（81260622）；重庆市教委科学技术研究项目资助（KJ1601007）；云南省应用基础研究计划青年项目（2014FD046）；云南省教育厅科学研究基金重点项目（2012Z119）