目的 研究黄芪-附子药对配伍对附子3种单酯型生物碱（苯甲酰次乌头原碱、苯甲酰新乌头原碱、苯甲酰乌头原碱）和3种双酯型生物碱（次乌头碱、新乌头碱、乌头碱）肠吸收的影响。方法 运用大鼠外翻肠囊模型，选择十二指肠、空肠、回肠为研究肠段，以表观渗透系数（P_app）为评价指标，考察黄芪对附子6种生物碱P_app的影响。结果 当附子-黄芪3∶1时，黄芪在十二指肠和回肠能显著降低双酯型生物碱的P_app，在3种肠段均能降低单酯型生物碱的P_app；当附子-黄芪1∶1时，除次乌头碱在回肠外，黄芪能显著降低双酯型生物碱的P_app；当附子-黄芪1∶3时，黄芪能显著降低单酯型生物碱（除回肠外）的P_app，在各肠段均能显著降低3种双酯型生物碱的P_app。结论 黄芪可抑制附子生物碱的吸收，且其抑制作用因配伍比例、生物碱的种类和肠段不同而不同。

Objective To study the effect of Radix Astragali and Aconiti Lateralis Radix Preparata on the intestinal absorption of the three kinds of monoester-type alkaloids (benzoylthioate, benzoyl neostearine, and benzoyl aconitine) and three kinds of diester-type alkaloids (hypaconitine, neaconitine, and aconitine) in Aconiti Lateralis Radix Preparata. Methods The rat duodenum, the jejunum, and the ileum were selected to study the intestinal segment. The apparent permeability coefficient (P_app) was used to evaluate the effect of Radix Astragali on the P_app of six kinds of alkaloids in Aconiti Lateralis Radix Preparata. Results When Aconiti Lateralis Radix Preparata and Radix Astragali was 3:1, Radix Astragali can significantly reduce the dipeptide alkaloid P_app in the duodenum and ileum and reduce the monoester-type alkaloid P_app in three kinds of intestinal segments; When Aconiti Lateralis Radix Preparata and Radix Astragali was 1:1, except in the ileum, Radix Astragali can significantly reduce the P_app of the diester-type alkaloid; When Aconiti Lateralis Radix Preparata and Radix Astragali was 1:3, Radix Astragali can significantly reduce the P_app of the monoester-type alkaloid (except the parenteral) and significantly reduced the P_app of the three diester-type alkaloids. Conclusion Radix Astragali can inhibit the absorption of aconite alkaloids, and its inhibition effect is different due to different compatibility ratios, types of alkaloids and intestinal segments.

国家自然科学基金资助项目（81660757）；江西省卫生计生委中医药科研课题（2016A021）