目的 观察补骨脂盐炙前后对肾阳虚大鼠肝肾功能及口腔唾液腺水通道蛋白5（AQP5）、结肠AQP4、肾脏AQP2基因表达的影响，分析阐明盐炙缓和补骨脂毒副作用的机制。方法 采用sc氢化可的松的方法复制大鼠肾阳虚模型，以化学试剂法测定大鼠血清中总蛋白（TP）、白蛋白（Alb）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、血肌酐（Scr）和血尿素氮（BUN）的量，荧光定量PCR（qRT-PCR）法测定口腔唾液腺AQP5、结肠AQP4、肾脏AQP2 mRNA的表达。结果 与模型组比较，补骨脂生品和盐炙品组大鼠血清中的ALT、AST量显著升高，Alb量显著降低（P<0.05）；补骨脂盐炙品组ALT、AST水平低于生品组；各给药组BUN及Scr水平显著高于模型组（P<0.05）。qRT-PCR结果显示，与对照组比较，模型组大鼠AQP2、AQP4 mRNA表达明显降低（P<0.05），AQP5的mRNA表达明显增加（P<0.05）；与模型组比较，补骨脂生品组大鼠AQP4 mRNA表达显著升高（P<0.05），AQP5 mRNA表达显著降低（P<0.05）；与补骨脂生品组比较，补骨脂盐炙品组AQP2、AQP5 mRNA表达明显升高（P<0.05），AQP4 mRNA表达明显降低（P<0.05）。结论 补骨脂盐炙后可缓解药物对肾阳虚大鼠肝肾功能的副作用；同时缓解生品的燥性，盐炙对药物燥性的缓解作用可能与其对体内AQP的基因表达调控有关。

Objective To observe the effect of raw and salt-processed of psoralen on the function of liver and kidney and the expression of aquaporins 5 (AQP5), AQP4, and AQP2 in the kidney-yang deficiency model rats. Methods Used hydrocortisone made deficiency of kidney-YANG model rats, total protein (TP), albumin (Alb) and aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum creatinine (Scr), and urea nitrogen (BUN) content in serum of rats were determined by chemical reagent method. The expression of AQP5, AQP4, and AQP2 were detected by qRT-PCR. Results The levels of ALT and AST in the raw and salt-processed of psoralen groups increased significantly, and Alb decreased significantly compared with model group (P < 0.05); The levels of ALT and AST in the salt-processed of psoralen group were lower than that in the raw psoralen group; the levels of BUN and Scr in the treated group were significantly higher than that in the model group (P < 0.05). The expression of mRNA for AQP2 and AQP4 of model group was decreased significantly (P < 0.05) compared with control group, and expression of AQP5 mRNA increased significantly (P < 0.05). The expression of AQP4 mRNA in raw psoralen group was significantly higher than that in model group (P < 0.05), and AQP5 mRNA was significantly lower than that in model group (P < 0.05). Moreover, the expression of AQP2 and AQP5 mRNA of salt-processed of psoralen group was significantly higher than that of the raw psoralen group (P < 0.05), and AQP4 mRNA expression was significantly lower than that of salt-processed of psoralen group (P < 0.05). Conclusion Salt-processed of psoralen can reduce the side effects of drugs on liver and kidney function in kidney-yang deficiency model rats, it can also alleviate the dryness of raw psoralen. The relief of dryness by salt-processed of psoralen may be related to the regulation of the gene expression of aquaporins in vivo.

国家自然科学基金资助项目（81303228）；四川省教育厅课题（13zb0306）；国家自然科学基金子课题（J1310034-32）；四川省教育厅青年基金项目（08ZB076）