目的 注释并分类红花Carthamus tinctorius中转录因子家族，分析其在不同组织间的表达水平差异。方法 使用红花不同组织转录组测序数据组装Unigene，利用Nr数据库中转录因子信息比对并注释红花转录因子，同时对MYB转录因子家族的基因表达、蛋白保守结构域及系统发育树等内容重点分析。结果 红花转录组测序数据中共获得731个转录因子，分属42个转录因子家族。MYB转录因子家族成员的表达及蛋白结构分析显示，MYB转录因子家族在花冠中表达差异显著，氨基酸序列中存在3个保守的蛋白核心结构，系统发育树分析显示聚为2类MYB亚族。结论 红花MADs-box、bHLH、MYB、AP2转录因子家族成员最多，在不同组织间表达差异显著，MYB转录因子家族具有一定保守性。

Objective This research aimed to analyze transcription factors (TFs) and their expression level in different tissues of Carthamus tinctorius. Methods Seed, leaf and petal transcriptome were analyzed by Solexa sequencing and assembled into Unigene. TFs were obtained according to Nr database. Gene expression, conservative Motif and evolutionary tree of MYB family were analyzed. Results A total of 731 transcription factors belong to 42 transcription factor family were obtained. Furthermore, MYB expressed differently in corolla when contrast to seed and leaf. There are three conserved protein domains in MYB family and MYB family was clustered into two subfamilies based on phylogenetic tree results. Conclusion TF families of MADs-box, WRKY, MYB and AP2 were differentially expressed significantly in various tissue samples and MYB TF family are conserved.

国家高技术研究发展计划（“863”）资助项目（2011AA100606）；国家自然科学基金项目（31501366）；吉林省科技厅项目（20150623024TC-11）；吉林农业大学科研启动基金（2015018）