目的 研究民族药隔山消提取物的神经氨酸酶（NA）抑制活性及活性部位的化学成分，并通过分子对接技术和NA抑制实验进一步探究其活性成分的NA抑制作用。方法 隔山消经石油醚渗漉、乙醇提取、醋酸乙酯萃取和水煎煮后，得到4个提取部位，采用荧光法测定4个提取部位NA抑制活性。应用UPLC-Q-Exactive技术快速归属和鉴定最佳活性部位中的化学成分；利用MOE 2014软件，以NA为靶点，对该活性部位中7个主要成分进行分子对接；再通过荧光法测定该7个成分的NA抑制活性。结果 隔山消醋酸乙酯部位NA抑制活性最佳，半数抑制浓度（IC₅₀）为137.31 μg/mL；高分辨质谱鉴定了该部位21个化学成分，包括酚酸类3个、黄酮类2个、鞣质类16个，其中7个化学成分经与对照品比对而准确鉴定，13个化合物在该植物中首次报道；分子对接实验表明五没食子酰葡萄糖、鞣花酸、原儿茶酸对接效果较好，进一步NA抑制验证实验表明7个化合物均有NA抑制活性，其中鞣花酸和五没食子酰葡萄糖效果最好，IC₅₀分别为426.67、440.28 μmol/L。结论 隔山消提取物具有NA抑制活性，鞣花酸和五没食子酰葡萄糖为其主要活性成分。

Objective To investigate the inhibitory activity of neuraminidase (NA) and the chemical constituents of the active fraction of the extract from Geranium strictipes, and further investigate the anti-NA activity of the components by molecular docking technique and NA inhibition test. Methods G. strictipes was extracted by petroleum ether, ethanol, ethyl acetate and water, and four extracts were obtained. The inhibition activity of NA was determined by fluorescence method and the inhibition rate was calculated. UPLC-Q-Exactive was used to rapidly identify and identify the chemical constituents in the most active sites. Using MOE 2014, seven main components were inserted to NA. And the NA inhibitory activity of these seven major components was determined by fluorescence method, and the inhibition rate was calculated. Results The results showed that the ethyl acetate was the most active with the IC₅₀ value of 137.31 μg/mL. Moreover, a total of 20 chemical constituents were identified by UPLC-Q-Exactive, including 3 phenolic acids, 2 flavonoids, and 16 tannins. Among them, seven chemical constituents were identified accurately with the standard and 13 compounds were reported for the first time in the plant. The results of molecular docking showed that pentagalloylglucose, ellagic acid and protocatechuic acid had better antagonistic effect. Further NA inhibition test showed that all the seven compounds had inhibitory activity, especially for the ellagic acid and pentagalloylglucose which had the best effect and IC₅₀ were 426.67 and 440.28 μmol/L, respectively. Conclusion The extract from G. strictipes have the inhibitory activity of NA, and the ellagic acid and pentagalloylglucose are main active compenents.

国家自然科学基金委面上项目——肉苁蓉自然基金（81473315）；西南民族大学研究生创新型科研项目（CX2017SZ095）；中国医学科学院医学与健康科技创新工程经费资助（2016-I2M-3-015）