目的 研究山楂原花青素（HPC）和维生素C（VC）联合应用对胰岛素抵抗（IR）大鼠肾脏损伤的保护作用。方法 采用高脂膳食法诱导IR大鼠，造模后继续高脂喂养2周，制备IR肾病大鼠模型，并检测空腹血糖、血清胰岛素以及尿液各项指标、微量白蛋白（mAlb）水平；将造模后的50只IR肾病大鼠分为模型组、HPC（56 mg/kg）组、VC（180 mg/kg）组、HPC（56 mg/kg）+VC（180 mg/kg）组和罗格列酮（2 mg/kg）组，另取10只正常大鼠作为对照组，各组均ig给药，持续20周，测定大鼠空腹血糖、胰岛素、尿糖水平，检测尿液中肌酐（Cr）、尿素氮（UN）、尿酸（UA）、总蛋白水平以及mAlb、N-乙酰-β-D-葡萄糖苷酶（NAG）24 h排泄量，光镜下观察肾脏组织病理学改变。结果 与对照组比较，模型组大鼠空腹血糖、血清胰岛素以及尿糖水平显著升高（P<0.01），尿中UN、UA、Cr水平显著升高（P<0.01），尿总蛋白、mAlb水平以及尿NAG排泄量均显著升高（P<0.01）。而HPC和VC联合应用后以上指标均显著下降（P<0.01），较HPC组和VC组作用显著（P<0.01），与罗格列酮组效果相近。HPC+VC组大鼠肾脏组织显微结构有明显改善。结论 HPC与VC联合应用可改善IR大鼠肾脏功能，对肾脏有一定的保护作用。

Objective To explore the effects of hawthorn proanthocyanidins (HPC) and vitamin C (VC) on kidney protection in insulin-resistance (IR) rats. Methods The IR rats were induced by high-fat diet in order to establish the model of nephropathy in type Ⅱ diabetic rats, and the high-fat feeding were continued for 2 weeks after the IR rats were made. The contents of fasting blood glucose, serum insulin, glucose and microabuminuria (mAlb) in urine were tested. Fifty IR model rats were divided into model, HPC (56 mg/kg), VC (180 mg/kg), combined application of HPC (56 mg/kg) and VC (180 mg/kg), and rosiglitazone (2 mg/kg) groups. Another 10 normal rats were selected randomly to be control group. After continuous ig administration for 20 weeks in each group, these indexs, such as the levels of fasting blood glucose (FBG), serum insulin (SI), glucose in urine (24 h), creatinine (Cr), urea nitrongen (UN), uric acid (UA), were all detected. The excretion of urinary total protein (UTP), microabuminuria (mAlb), N-acetyl-β-D-glucosaminidase (NAG) were also measured. Moreover, the histopathological microstructure of kidney was observed by light microscope. Results After modeling, the levels of FBG, SI and glucose in urine increased significantly (P < 0.01) in comparation with the control group, so does the levels of UN, Cr, UA (P < 0.01) and the excretion rates of UTP, mAlb, NAG (P < 0.01). However, all these indexs were decreased significantly (P < 0.01) after the combined use of HPC and VC, which were more notable than that in HPC and VC group (P < 0.01) and equivalent to that in rosiglitazone group. Conclusion The combined application of HPC and VC can improve the renal function of IR rats and have protective effects on kidney injury.

国家自然科学基金资助项目（81302423）；山东省高校科技计划项目（J17KA224）