目的 研究金银花（忍冬Lonicera japonica干燥花蕾）的化学成分。方法 反复利用硅胶柱色谱、Sephadex LH-20、中压柱色谱及制备液相色谱等方法分离纯化；通过MS、¹H-NMR及¹³C-NMR等技术鉴定化合物结构。结果 从金银花醋酸乙酯部位分离得到12个化合物，分别鉴定为对羟基苯甲酸（1）、香草酸（2）、槲皮素-3-O-α-L-吡喃鼠李糖苷（3）、山柰酚-3-O-β-D-吡喃葡萄糖苷（4）、3'，4'，7-三羟基-3，5-二甲氧基黄酮（5）、异鼠李素（6）、金丝桃苷（7）、槲皮素-7-O-β-D-吡喃葡萄糖苷（8）、环裂马钱子酸（9）、断氧马钱子苷半缩醛内酯（10）、5-O-咖啡酰奎宁酸甲酯（11）、豆甾醇（12）。结论 化合物5首次从忍冬属植物中分离得到；化合物1~4、6、11、12首次从金银花中分离得到。

Objective To investigate the chemical constituents of the flower buds of Lonicera japonica. Methods The chemical constituents were isolated by repeated silica gel chromatography, Sephadex LH-20, medium pressure column chromatography and preparative HPLC, and their structures were elucidated by spectroscopic analyses and comparison of MS, ¹H-NMR and ¹³C-NMR data with those reported in literature. Results Twelve compounds from the EtOAc fraction of L. japonica included p-hydroxybenzoic acid (1), vanillic acid (2), quercetin-3-O-α-L-rhamnopyranoside (3), kaemnpferol-3-O-β-D-glucopyranoside (4), 3',4',7-trihydroxy-3,5-dimethoxyflavone (5), isorhamnetin (6), hyperoside (7), quercetin-7-O-β-D-glucopyranoside (8), secologanic acid (9), vogeloside (10), 5-O-caffeoylquinic acidmethylester (11) and stigmasterol (12). Conclusion Compound 5 is obtained from the plant in Lonicera Linn. for the first time; Compounds 1-4, 6, 11 and 12 are obtained from this plants for the first time.

科技部重大新药创制：现代中药创新集群与数字制药技术平台（2013ZX09402203）