目的 比较评价桃仁-大黄药对（PS-RRR）不同配比对急性血瘀模型大鼠血液流变学和凝血功能的影响，揭示PS-RRR活血化瘀效应相互作用的范围、性质与程度。方法 采用冰水浴与注射盐酸肾上腺素方法复制大鼠急性血瘀模型，给予不同配比（0：1、1：5、2：5、2：3、1：1、3：2、5：2、5：1、1：0）PS-RRR后，通过测定全血黏度（WBV）、血沉（ESR）、红细胞聚集指数（EAI）、凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）、纤维蛋白原（FIB）、凝血酶时间（TT），观察其对血瘀大鼠血液流变学、凝血指标的影响。采用响应曲面分析法和多指标综合指数法对PS-RRR不同配比的总活血化瘀效应进行综合比较分析。结果 PS-RRR配比在2：3~3：2表现出明显的协同作用（协同作用强度为-0.8）；在桃仁（PS）剂量5.5~10 g，大黄（RRR）剂量2.1~5.8 g，表现出拮抗作用（拮抗作用强度最大为0.6）；而其他比例并未表现出明显的协同或者拮抗作用。结论 初步揭示了PS-RRR活血化瘀效应相互作用的范围、性质与程度，且PS-RRR活血化瘀作用的最佳配比与临床中医方剂中使用PS-RRR配比1：1频次最高的结论相一致。

Objective To evaluate Persicae Semen-Rhei Radix et Rhizoma (PS-RRR) of different compatibility of acute blood stasis rats blood rheology and blood coagulation function, and to reveale the effect of PS-RRR for promoting blood circulation to remove blood stasis effect scope, nature, and degree of interaction. Methods With ice water bath and injected adrenaline hydrochloride copy of acute blood stasis rats model, different ratio (0:1, 1:5, 2:5, 2:3, 1:1, 3:2, 5:2, 5:1, and 1:0) of different concentration of PS-RRR was given for later. Through the determination of whole blood viscosity (WBV), blood sedimentation (ESR), erythrocyte aggregation index (EAI), prothrombin time (PT), partial thrombin (APTT), fibrinogen (FIB) content of coagulation, thrombin time (TT), and the blood rheology of blood stasis rats was observed, the influence of the blood coagulation indexes. Then response surface analysis and multi-index comprehensive index method of PS-RRR different compatibility of promoting blood circulation was used to remove blood stasis effect comprehensive comparative analysis. Results The ratio of PS-RRR between 2:3 to 3:2 showed obvious synergy (strength of synergy:-0.8); In PS dose from 5.5-10 g and RRR dose from 2.1-5.8 g area showed the antagonism function (antagonism effect strength maximum:0.6); While other percentage did not show obvious synergy or antagonism. Conclusion The results of reveal that scope, nature, and degree of PS-RRR for promoting blood circulation to remove blood stasis interaction effect, the and its prescriptions of traditional Chinese medicine (TCM) and clinical use of PS-RRR 1:1 is consistent with the highest frequency of conclusion. PS-RRR provides scientific basis for clinical applications.

国家自然科学基金资助项目（30901970）；陕西省教育厅项目（14JK1204）；陕西省教育厅重点实验室项目（13JS030）