目的 研究黄秋葵提取物对高脂饮食和链脲佐菌素（STZ）诱导的2型糖尿病小鼠的降糖作用及与肿瘤坏死因子-α（TNF-α）和胰岛素降解酶（IDE）的相关性。方法 采用高脂饲料喂养，并ip STZ制备小鼠2型糖尿病模型，随机分为对照组，模型组，二甲双胍组，黄秋葵醇提物（HQK）高、中、低剂量组，黄秋葵水提物（WQK）高、中、低剂量组，均ig给药。给药40 d后进行空腹血糖、口服葡萄糖糖耐量（OGTT）测定、血清和肝组织中相关生化指标检测，并对肝组织进行HE染色。通过荧光定量PCR探讨黄秋葵提取物降糖活性与TNF-α、IDE基因表达的相关性。结果 HQK高剂量组和WQK中剂量组显著降低小鼠空腹血糖，并改善口服葡萄糖耐受量，有效抑制2型糖尿病小鼠血清中总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-C）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和肝组织中AST、ALT，并抑制肝脏中TNF-α、IDE的mRNA表达量，明显提升血清中高密度脂蛋白胆固醇（HDL-C）。肝组织HE病理观察显示HQK高剂量组和WQK中剂量组对肝细胞有一定的保护作用。结论 HQK和WQK通过降低2型糖尿病模型小鼠的TC、TG、LDL-C，同时升高HDL-C来改善小鼠的肥胖、高血脂症状，对2型糖尿病引起的肝损伤有一定的保护作用，并且降血糖与肝脏保护作用与TNF-α和IDE的基因表达量有一定的相关性。

Objective To analyze the hypoglycemic effect of okra (Abelmoschus esculentus) extract on the hyperlipidemia and streptozotocin (STZ)-induced type 2 diabetic mice and the correlation between TNF-α and IDE. Methods The diabetic mice were induced by high fat diet followed by a low dose of streptozotocin (STZ) and randomly divided into control group, diabetic model group, okra alcohol extract high, medium, and low dose (400, 200, and 100 mg/kg) groups, okra aqueous extract high, medium, and low dose (200, 100, and 50 mg/kg) groups. After the exposure to conventional or different treatment groups for 40 d, fasting blood sugar, glucose tolerance, related biochemical indexes in serum and liver tissue, and liver HE staining pathological sections were measured. Results The okra alcohol extract high dose group and okra aqueous extract middle dose group effectively reduced the mice fasting blood glucose and improved oral glucose tolerance in mice, inhibited of type 2 diabetes mellitus rats serum TC, TG, LDL-C, ALT, AST, and mRNA expression of TNF-α and IDE in the rats liver. Conclusion The okra alcohol extract with high dose and the okra aqueous extract with middle dose could reduce TC, TG, LDL-C, while increase HDL-C to improve the mouse obesity, and hyperlipidemia symptoms. And hypoglycemic and liver protection and TNF-α and IDE gene expression levels have a certain correlation.

吉林省自然科学基金项目（20160101017JC）；吉林省科技发展计划项目，重点科技攻关（20170204023NY）；吉林省大学生创业资金项目（20160521011HJ）