目的 建立一测多评法（QAMS）同时测定苦丁茶冬青Ilex kudingcha中6种成分的方法。方法 采用液质联用法确定了供试品中山柰酚-3-O-β-D-芸香糖苷、异鼠李素-3-O-β-D-芸香糖苷、苦丁冬青皂苷C、苦丁冬青皂苷A、苦丁冬青皂苷E、苦丁冬青皂苷D的质量分数。采用HPLC法，以苦丁冬青皂苷C为内参物，测定了另外5种成分的相对校正因子，并利用相对校正因子计算其他5种成分的量。同时采用外标法（ESM）对这6种成分进行测定，比较ESM和QAMS的差异，验证QAMS的可行性及准确性。结果 利用QAMS法计算得到的苦丁茶冬青中6种成分的量与ESM测定值之间无显著性差异。结论 采用QAMS测定苦丁茶冬青中6种成分的量简便、可行、准确。

Objective To establish a quantitative analysis of multi-components by single marker (QAMS) for the simultaneous determination of six components in Ilex kudingcha by HPLC. Methods The purity of kaempferol-3-O-β-D-rutanoside, isorhamnetin-3-O-β-D-rutanoside, kudinoside C, kudinoside A, kudinoside E, and kudinoside D was determined by HPLC-MS in test sample taking kudinoside C as internal standard, relative correction factors of the other five compounds were determined, and their contents were calculated. At the same time, the contents of these six components were determined by external standard method (ESM), and the difference between ESM and QAMS was compared to verify the feasibility and accuracy of QAMS method. Results There was no significant difference of the six components' contents calculated between QAMS method and ESM method. Conclusion It is convenient, feasible, and accurate to measure the content of six components in I. kudingcha by QASM method.

吉林省科技发展计划项目（20150311037YY）；吉林省卫生计生科研计划项目（2014Z206）；吉林省中医药科技项目（2014-ZD28）