目的 从代谢通路角度阐明湿困脾胃证的本质及藿香正气口服液的干预作用。方法 采用综合物理造模方法制备湿困脾胃证大鼠模型。测量体质量、体长、尾长、腹围指数等宏观体征指标和自主活动次数。利用核磁共振氢谱（¹H-NMR）技术建立大鼠血清和粪便的代谢物谱图，分析藿香正气口服液给药后湿困脾胃证大鼠相关潜在生物标志物的变化。结果 与对照组比较，模型组大鼠体质量降低，体长增长缓慢，腹围指数增加，自主活动次数减少；血清和粪便代谢物组发生显著改变。藿香正气口服液能改善模型大鼠宏观体征和行为学指标。同时，藿香正气口服液能对湿困脾胃证大鼠血清和粪便代谢紊乱产生有效的干预，并分别使血清和粪便中与湿困脾胃证相关的6个和8个潜在生物标志物产生显著地回调。结论 藿香正气口服液通过调节肠道菌群及逆转机体能量代谢和氨基酸代谢紊乱来干预大鼠湿困脾胃证。

Objective To classify the essence of dampness obstructing spleen-stomach syndrome (DOSS) in rats and the effect of Huoxiang Zhengqi Oral Liquid (HZOL) on DOSS. Methods Comprehensive physical methods were applied to establish the rat DOSS model. Body weight, body length, tail length, abdominal girth index, and locomotor activity were observed. Moreover, a proton nuclear magnetic resonance (¹H-NMR) based metabonomic approach was developed to profile DOSS-related metabolic perturbations in rat serum and feces and to investigate the intervention effect of HZOL in rats. Results Compared with the healthy control group, rats with DOSS showed weight loss, slow growth of body length, increased abdominal girth index, and decreased locomotor activity along with metabolic perturbations in serum and feces. The disturbed metabolic state associated with DOSS in rat serum and feces could be restored by HZOL. Meanwhile, six and eight potential biomarkers related to DOSS in rat serum and feces, respectively, were reversed by administration of HZOL. Conclusion The current study demonstrated that HZOL exerted protective effect against DOSS in rats, which was probably mediated by amelioration of disturbance in energy and amino acid metabolism and gut microbiota.

国家自然科学基金青年基金项目（81603397，81403075）