目的 建立杜仲Eucommia ulmoides雄花6种活性成分的测定方法，分析杜仲雄花生长发育动态及活性成分的变化规律。方法 采用超声法进行提取，采用AlCl₃比色法测定总黄酮，采用HPLC法测定其他6种成分，色谱柱为Thermo hypersil gold柱（250 mm×4.6 mm，5 μm），以甲醇-0.5%磷酸水溶液为流动相，梯度洗脱；检测波长：206 nm（0~15 min）、236 nm（15~55 min）、255 nm（55~100 min）；体积流量1.0 mL/min。结果 花径、花高、鲜质量、干质量、雄蕊长度和雄蕊数均在盛花期达到最大值；总黄酮、绿原酸以及活性成分总量均以花蕾期最高，始花期最低；桃叶珊瑚苷量总体呈下降趋势；京尼平苷酸量花蕾期最低，至盛花期达到最高值，末花期有所下降；京尼平苷、异槲皮苷和紫云英苷量均以盛花期最高，始花期最低。结论 花期对杜仲雄花形态、产量、活性成分量及质量均有很大影响，为杜仲雄花质量控制及相关产品开发提供了重要依据。

Objective To establish a method for the determination of six active ingredients in Eucommia ulmoides male flowers, and analyze the dynamic changes of male flower development and active ingredients content of E. ulmoides. Methods The active ingredients were extracted by the ultrasound-assisted process, the content of total flavonoids was determined by AlCl₃ colorimetric method and other six ingredients were determined by HPLC, the samples were separated on a Thermal hypersil gold column (250 mm × 4.6 mm, 5 μm) with gradient elution of methanol-0.5% phosphoric acid at a flow rate of 1.0 mL/min, the UV wavelength was set at 206 nm (0-15 min), 236 nm (15-55 min), and 255 nm (55-100 min). Results Flower diameter, flower height, fresh weight, dry weight, stamen length and stamen number all reached the maximum at full bloom stage; The contents of total flavonoids, chlorogenic acid and total active ingredients were the highest at the bud stage and the lowest at the initial flowering stage; The content of aucubin showed a tendency of graudal decreasement; The content of geniposidic acid was the lowest at the bud stage and reached the highest at full bloom stage, and decreased at the end of flowering phase; The content of geniposide, isoquercitrin and astragaline were the highest at full bloom stage and the lowest at the initial flowering stage. Conclusion Flowering stage had a great impact on morphology, yield, active ingredients content and quality of E. ulmoides male flowers, which could provide important information for quality control and product development of E. ulmoides male flowers.

国家“十二五”农村领域科技计划课题研究任务合约（2012BAD21B0502）