目的 建立防己黄芪汤（FHD）HPLC-UV/ELSD指纹图谱分析方法，归属和指认复方中主要特征峰。方法 采用HPLC法，色谱柱为Venusil MP C₁₈（250 mm×4.6 mm，5 μm）；流动相为乙腈-0.1%甲酸水溶液（梯度洗脱），紫外检测器：检测波长为254 nm，蒸发光散射检测器：漂移管温度为110 ℃，空气体积流量为3 L/min；进样量10 μL；体积流量为1 mL/min。运用《中药色谱指纹图谱相似度评价系统（2008版）》对10批FHD指纹图谱进行相似度计算，并对共有峰进行药材归属及成分指认。结果 建立了FHD指纹图谱；10批FHD指纹图谱相似度良好；在FHD HPLC-UV指纹图谱中标定了20个共有峰，其中3、6~8号峰来自防己，1、2、4、5、9、12、13、16、20号峰来自黄芪，10、11、13、14、15、17~19号峰来自甘草。在FHD HPLC-ELSD指纹图谱中标定了16个共有峰，其中1'~3'号峰来自防己，4'、7'、9'、11'、13'、15'、16'号峰来自黄芪，5'~8'、10'、12'、14'号峰来自甘草。通过进样对照品、复方以及在复方中加对照品的方式指认出复方中9个成分，分别为9/4'号峰（毛蕊异黄酮葡萄糖苷）、11/6'号峰（甘草苷）、13/7'号峰（芒柄花苷）、15号峰（甘草素）、16/9'号峰（毛蕊异黄酮）、20/15'号峰（芒柄花素），11'号峰（黄芪甲苷）、13'号峰（黄芪皂苷II）、16'号峰（黄芪皂苷I）。结论 首次建立了FHD HPLC-UV/ELSD指纹图谱分析方法，该法操作简单、准确，精密度、重复性好，可用于表征FHD中化学成分信息，为FHD质量控制提供了可靠的科学依据。

Objective HPLC-UV/ELSD fingerprint was developed to assign and identify the main characteristic peaks in Fangji Huangqi Decoction (FHD). Methods High performance liquid chromatography: Venusil MP C₁₈ column (250 mm × 4.6 mm, 5 μm) was used with a gradient mobile phase system of acetonitrile-water containing 0.1% formic acid. Ultraviolet detector: the detection wavelength was 254 nm; Evaporative light scattering detector: The temperature of drift tube was maintained at 110 ℃ and the flow rate of air was 3 L/min. The injection volume was 10 μL; The flow rate was 1 mL/min. The similarities between the HPLC-UV/ELSD fingerprints of 10 batches of FHD extracts were calculated by similarity evaluation software, and the common peaks were assigned and identified simultaneously. Results The fingerprint of FHD was established. The complementarity among fingerprints of FHD was analyzed, showing a good correlation among 10 batches of FHD. The HPLC-UV fingerprint of FHD including 20 mutual peaks, of which four mutual peaks were from Stephania Tetrandra Radix, nine peaks were from Astragali Radix, eight peaks were from Glycyrrhizae Radix. The HPLC-ELSD fingerprint of FHD included 16 mutual peaks, of which three mutual peaks were from Stephania Tetrandra Radix, seven peaks were from Astragali Radix, seven peaks were from Glycyrrhizae Radix. Nine components were identified by comparison with the reference substance, FHD and adding the reference substance in FHD, which were calycosin-7-glucoside, liquiritin, ononin, liquirtigenin, calycosin, formononetin, astragaloside IV, astragaloside II, and astragaloside I. Conclusion It is the first time to establish the HPLC-UV/ELSD fingerprint of FHD. The method is simple, accurate, and reproducible, which can be used to characterize the chemical composition information of FHD and provide a scientific evidence for the quality control of FHD.

国家自然科学基金资助项目（3157010673）：多效中药黄芪的定向药效化学成分研究