目的 研究广西毛冬青Ilex pubescens var.kwangsiensis根的化学成分。方法 利用反复硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法、中压柱色谱法、高压快速制备色谱及半制备高效液相色谱等方法分离纯化，通过理化常数和NMR、MS波谱等方法鉴定化合物结构。结果 从广西毛冬青甲醇提取物中分离得到14个化合物，分别鉴定为丁香苷（1）、3，4-二羟基苯乙醇（2）、橄榄苦苷（3）、红景天苷（4）、木樨榄苷-11-甲酯（5）、（8E）-女贞子苷（6）、（8Z）-ligstroside（7）、oleoacteoside（8）、oleoside dimethyl ester（9）、olivil-4'-O-β-D-glucoside（10）、（+）-cyclo-olivil-6-O-β-D-glucoside（11）、（+）-cyclo-olivil-4'-O-β-D-glucoside（12）、ligstroside（13）、wilfordiol B（14）。结论 化合物2、4、12~14为首次从冬青属植物中分离得到，化合物1、3、5~11为首次从本植物中分离得到。

Objective To investigate the chemical constituents from the roots of Ilex pubescens. Methods The chemical constituents were isolated and purified by repeated silica gel column chromatography, Sephadex LH-20 gel column chromatography, medium pressure column chromatography, high pressure flash chromatography, and semi-preparative HPLC, and their structures were elucidated on the basis of physico-chemical constants and spectral analysis. Results Fourteen compounds were identified as syringin (1), 3,4-dihydroxyphenyl ethanol (2), oleuropein (3), salidroside (4), oleoside 11-methyl ester (5), (8E)-nuezhenide (6), (8Z)-ligstroside (7), oleoacteoside (8), oleoside dimethyl ester (9), olivil-4'-O-β-D-glucoside (10), (+)-cyclo-olivil-6-O-β-D-glucoside (11), (+)-cyclo-olivil-4'-O-β-D-glucoside (12), ligstroside (13), and wilfordiol B (14). Conclusion Compounds 2, 4, and 12-14 are obtained from this genus for the first time, and compounds 1, 3, and 5-11 are obtained from this plant for the first time.