目的 建立一测多评法（QAMS）测定类叶牡丹Caulophyllum robustum中cauloside G、cauloside H、leonticin D、cauloside D、cauloside B和cauloside C的量。方法 以类叶牡丹皂苷成分cauloside G为内参物，计算该成分与cauloside H、leonticin D、cauloside D、cauloside B和cauloside C的相对校正因子，并用获得的相对校正因子计算指标性皂苷的量，实现QAMS。同时用外标法测定药材中6种成分的量，考察QAMS的可行性。结果 相对校正因子的重现性良好，8个产地的17批药材的QAMS计算值与外标法实测值没有显著性差异。结论 在对照品缺乏的情况下，QAMS可用于类叶牡丹药材的质量评价，方法准确、可靠。

Objective To establish a method of quantitative analysis of multi-components by single-marker (QAMS) for simultaneous determining six saponins in Caulophyllum robustum. Methods Cauloside G was used as the internal reference standard; The relative correction factors of cauloside H, leonticin D, cauloside D, cauloside B, and cauloside C in C. robustum were calculated. To achieve the QAMS, the contents of indicative saponins were calculated by relative correction factors. At the same time, the external standard method was used to detect the contents of six kinds of ingredients in medicinal materials, and feasibility of QAMS was verified. Results The relative correction factor had good reproducibility, there were no significant differences among the calculated values of multi-evaluation and measured values of the external standard method. Conclusion In the absence of reference substance situation, QAMS can be used for the quality evaluation of C. robustum, and the method is accurate and reliable.

国家“十二五”重大新药创制项目（2013ZX09102019）；国家自然科学基金资助项目（2013ZX09102019）；黑龙江省自然科学基金资助项目（H201304）；黑龙江省教育厅创新人才支持计划（051591）