目的 研究野鸦椿Euscaphis japonica根抑制肝脂堆积活性部位及其化学成分。方法 萃取法制备不同极性部位，以油酸诱导的HepG2细胞脂堆积模型、油红O染色观察细胞内脂滴的堆积，并检测细胞内三酰甘油（TG）的量筛选抑制肝脂堆积活性部位，并采用硅胶、Sephadex LH-20、HPLC等多种色谱技术对活性部位进行分离纯化，根据理化性质和波谱数据鉴定化合物结构。结果 石油醚部位能显著抑制油酸诱导的HepG2细胞内脂滴的堆积并降低TG的量，从中分离得到9个化合物，分别鉴定为3β，19-二羟基-24-反式-阿魏酰基-熊果烷-12-烯-28-酸（1）、β-谷甾醇（2）、7-羟基-2-辛烯-5-内酯（3）、3，3'-二甲氧基-鞣花酸（4）、香草醛（5）、5-羰基-四氢呋喃-3-甲酸乙酯（6）、没食子酸（7）、3，3'-二甲氧基-鞣花酸-4-（5"-乙酰基）-α-L-阿拉伯糖苷（8）、佛手柑内酯（9）。结论 石油醚部位为野鸦椿根抑制肝脂堆积活性部位，化合物1、6、8和9为首次从该属植物中分离得到。

Objective To screen the active fraction of Euscaphis japonica roots with inhibitory activity on hepatic lipid accumulation and investigate its chemical constituents. Methods Different polar fractions were prepared by extraction with organic solvents, oil Red O staining and triglyceride content assay were used to determine inhibitory activity of different polar fraction on oleic acid induced triglyceride accumulation on HepG2 cells, and the constituents of active fraction were isolated and purified by various chromatography techniques such as column chromatography on silica gel, Sephadex LH-20, and HPLC, and their structures were identified by physicochemical properties and spectral data. Results The petroleum ether fraction exhibited significantly inhibitory activity on oleic acid induced triglyceride accumulation on HepG2 cells. Nine compounds were isolated and identified as 3β,19- dihydroxy-24-trans-ferulyloxyurs-12-en-28-oic acid (1), β-sitosterol (2), 7-hydroxy-2-octen-5-olide (3), 3,3'-dimethoxy-ellagic acid (4), vanillin (5), ethyl-5-oxo-tetrahydro-3-furancarboxylate (6), gallic acid (7), 3,3'-di-O-methylellagic acid 4-(5"-acetyl)-α-L- arabinofuranoside (8), and bergapten (9). Conclusion The petroleum ether fraction is main active fraction. The compounds 1, 6, 8, and 9 are obtained from genus Euscaphis Sieb. et Zucc. for the first time.

国家自然科学基金资助项目（81560703）；湖北省自然科学基金项目（2016CFB508）