目的 探讨啤酒花提取物对原代棕色脂肪细胞增殖和功能的影响及潜在机制，为2型糖尿病和肥胖症的治疗提供新依据。方法 体外培养新生鼠棕色脂肪前体细胞，添加不同质量浓度啤酒花提取物，用MTT法检测细胞的增殖；诱导分化成熟后，用油红O染色以染色比色法分析细胞脂滴消耗程度；采用Western blotting技术检测相关蛋白表达水平，包括解耦联蛋白1（UCP1）、p38α丝裂原激活蛋白激酶（p38αMAPK）、过氧化物酶体增殖活化受体γ辅助活化因子1α（PGC1α）。结果 啤酒花提取物对原代前棕色脂肪细胞的增殖没有影响；与对照组比较，随着质量浓度的增加，啤酒花提取物可以显著提高脂滴消耗率；啤酒花提取物能够使棕色脂肪细胞中UCP1、p38αMAPK和PGC1α蛋白表达水平上升。结论 啤酒花提取物可以提高棕色脂肪细胞产热功能，加强能量代谢，其可能机制是增强p38MAPK-PGC1α-UCP1级联反应，进而提高棕色脂肪特异性蛋白UCP1的表达。

Objective To investigate the effects of Humulus lupulus extract on the function of primary brown preadipocyte and its mechanisms, and try to provide new treatment for obesity and type 2 diabetes. Methods Primary brown adipocytes in newborn rats were cultivated in vitro. After adding different doses of H. lupulus extract, MTT method was used to detect the cell proliferation, and oil red O staining method was used to analyze the lipid droplets consumption rate of H. lupulus extract on the brown adipocyte. The proteins expression of brown adipocytes (such as UCP1, p38αMAPK, and PGC1α) in different groups was detected by Western blotting. Results The cell proliferation of primary brown preadipocytes was not effected by H. lupulus extract. Compared with control group, with the increasing dose, the lipid droplets consumption rate of H. lupulus extract on the brown adipocyte was increased. The expression of UCP1, p38αMAPK, and PGC1α was upregulated compared with the control group. Conclusion H. lupulus extract may improve the heat production of brown adipocytes, and strengthen the energy metabolism. The possible mechanism may via enhancing p38MAPK-PGC1α-UCP1 cascade reaction, sequentially increasing the expression of brown fat specificity protein UCP1.

后勤科研目录项目重大专项（AWS14L008）