目的 建立HPLC-DAD法同时测定参芪十一味颗粒（SSG）中23-乙酰泽泻醇B、阿魏酸、毛蕊花糖苷、人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁、天麻素、大黄酚、橙黄决明素、毛蕊异黄酮葡萄糖苷和金丝桃苷的方法。方法 采用RP-HPLC法，色谱柱为Waters XBridge-C₁₈（250 mm×4.6 mm，5.0 μm）；流动相为甲醇-乙腈-水（15∶80∶5）和乙腈-0.1%磷酸水溶液（10∶90），梯度洗脱，体积流量1.0 mL/min；柱温35 ℃，进样量10 μL。结果 23-乙酰泽泻醇B、阿魏酸、毛蕊花糖苷、人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁、天麻素、大黄酚、橙黄决明素、毛蕊异黄酮葡萄糖苷和金丝桃苷11种成分能够达到很好分离；其线性范围分别为0.4~8.0 μg/mL（r=0.999 2）、0.2~4.0 μg/mL（r=0.999 5）、0.2~4.0 μg/mL（r=0.999 5）、0.1~2.0 μg/mL（r=0.999 6）、0.1~2.0 μg/mL（r=0.999 7）、0.1~2.0 μg/mL（r=0.999 4）、0.5~10 μg/mL（r=0.999 2）、0.6~12 μg/mL（r=0.999 2）、0.4~8.0 μg/mL（r=0.999 4）、1.0~20 μg/mL（r=0.999 6）、0.8~16 μg/mL（r=0.999 3），平均加样回收率分别为98.1%、98.1%、99.1%、98.3%、99.5%、99.9%、98.5%、100.4%、101.6%、99.7%、101.2%，RSD分别为0.9%、1.6%、1.6%、1.8%、1.5%、0.6%、0.7%、0.8%、0.4%、0.9%、1.1%（n=6）。9批次供试品中23-乙酰泽泻醇B、阿魏酸、毛蕊花糖苷、人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁、天麻素、大黄酚、橙黄决明素、毛蕊异黄酮葡萄糖苷和金丝桃苷质量浓度分别为0.081~0.089、0.261~0.269、0.060~0.069、0.038~0.047、0.030~0.037、0.042~0.049、0.420~0.428、0.141~0.151、0.178~0.189、0.107~0.117、0.069~0.078 mg/g，结果表明本品各批次之间差异较小。结论 本方法操作简便，测定结果准确可靠，可用于SSG的质量控制。

Objective To establish HPLC-DAD method for the simultaneous determination of 23-acetate alisol B, ferulic acid, verbascoside, ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁, gastrodin, chrysophanol, aurantio-obtusin, calycosin 7-O-β-D-glucopyranoside, and hyperoside in Shenqi Shiyiwei Granule (SSG). Methods The chromatographic separation was achieved on an Waters XBridge-C₁₈ (250 mm×4.6 mm, 5.0 μm) column with methanol-acetonitrile-water (15:80:5) and methanol-0.1% phosphoric acid (10:90) as mobile phases for gradient elution, at the flow rate of 1.0 mL/min; The column temperature was 35℃.Results The linear ranges of 23-acetate alisol B, ferulic acid, verbascoside, ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁, gastrodin, chrysophanol, aurantio-obtusin, calycosin 7-O-β-D-glucopyranoside, and hyperoside were 0.4-8.0 μg/mL (r=0.999 2), 0.2-4.0 μg/mL (r=0.999 5), 0.2-4.0 μg/mL (r=0.999 5), 0.1-2.0 μg/mL (r=0.999 6), 0.1-2.0 μg/mL (r=0.999 7), 0.1-2.0 μg/mL (r=0.999 4), 0.5-10 μg/mL (r=0.999 2), 0.6-12 μg/mL (r=0.999 2), 0.4-8.0 μg/mL (r=0.999 4), 1.0-20 μg/mL (r=0.999 6), and 0.8-16 μg/mL (r=0.9993). The average recoveries (n=6) were 98.1% (RSD=0.9%), 98.1% (RSD=1.6%), 99.1% (RSD=1.6%), 98.3% (RSD=1.8%), 99.5% (RSD=1.5%), 99.9% (RSD=0.6%), 98.5% (RSD=0.7%), 100.4 (RSD=0.8%), 101.6% (RSD=0.4%), 99.7% (RSD=0.9%), and 101.2% (RSD=1.1%), respectively. The contents of nine batches of 23-acetate alisol B, ferulic acid, verbascoside, ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁, gastrodin, chrysophanol, aurantio-obtusin, calycosin 7-O-β-D-glucopyranoside, and hyperoside were 0.081-0.089, 0.261-0.269, 0.060-0.069, 0.038-0.047, 0.030-0.037, 0.042-0.049, 0.420-0.428, 0.141-0.151, 0.178-0.189, 0.107-0.117, and 0.069-0.078 mg/g. The results showed that there was little difference among the batches. Conclusion The method is accurate, sensitive, credible, and repeatable. It can be applied to the quality control of SSG.