目的 建立五子衍宗方（WYP）制剂的HPLC指纹图谱，对其中的特征峰进行指认，并对WYP制剂-药材进行谱峰匹配研究。方法 采用HPLC-DAD检测法，色谱柱为Waters Symmetry^® C₁₈（250 mm×4.6 mm，5 μm），流动相为0.4%磷酸水溶液-乙腈，体积流量1 mL/min，线性梯度洗脱，柱温35℃，检测波长为254 nm，对12个批次的WYP样品进行HPLC指纹图谱相似性评价，并将WYP对照图谱与单味药材进行谱峰匹配。结果 在12批WYP样品色谱图中共有24个共有峰，指认的特征峰有8个，分别为没食子酸（1号峰）、京尼平苷酸（4号峰）、绿原酸（8号峰）、金丝桃苷（19号峰）、异槲皮苷（20号峰）、毛蕊花糖苷（21号峰）、山柰酚-3-O-芸香糖苷（22号峰）和异毛蕊花糖苷（23号峰），单味药材色谱峰与WYP谱峰共有峰匹配数分别为枸杞子8个（2、3、6、7、8、11、12、18号峰）、菟丝子10个（6、7、8、9、10、15、16、19、20、24号峰）、覆盆子7个（1、3、7、13、14、17、22号峰）、车前子3个（4、21、23号峰）、五味子1个（3号峰）。各批WYP样品之间的相似度均大于0.959，各批WYP样品与对照指纹图谱之间的相似度均大于0.979。结论 建立的WYP指纹图谱相似度较高，同时结合制剂用药材与WYP制剂指纹图谱谱峰匹配相结合的方法为WYP的定性、定量鉴别提供了科学、简便可行的评定方法。

Objective To establish the HPLC-DAD fingerprints of Wuzi Yanzong Prescription (WYP) and to identify the characteristic peaks ion and preparations-medicinal materials peak pattern matching. Methods The HPLC-DAD analysis was carried out using acetonitrile-0.4% phosphate acid aqueous solution at the flow out on a Waters Symmetry^® C₁₈ column (250 mm×4.6 mm, 5 μm), using a mobile phase of rate of 1.0 mL/min. The detection wavelength was set at 254 nm and column temperature was maintained at 35℃. Similarity on 12 batches of WYP was estimated, and peak pattern matching of the original medicine was conducted. Results The results showed that 24 common peaks were defined as follows:The peaks No. 1 was gallic acid, No. 4 was geniposidic acid, No. 8 was chlorogenic acid, No.19 was hyperin, No. 20 was isoquercitrin, No. 21 was acteoside, No. 22 was kaempferol 3-rutinoside, and No. 23 was isoacteoside. The peak pattern matching showed that WYP had 8 (No. 2, 3, 6, 7, 8, 11, 12, and 18), 10 (No. 6, 7, 8, 9, 10, 15, 16, 19, 20, and 24), 7 (No. 1, 3, 7, 13, 14, 17, and 22), 3 (No. 4, 21, 23), and 1 (No. 3) matching peaks with Lycium barbarum, Cuscuta chinensis, Rubus chingii, Plantago asiatica, and Schisandra chinensis, respectively. The fingerprint similarity among the samples was all over 0.959. The fingerprint similarity between the chromatographic control fingerprint and samples was all over 0.979. Conclusion Similarity evaluation combined with the peak matching of fingerprint for WYP could provide the scientific and simplicity methods for its identification and quality control.

河北省自然科学基金资助项目（H2012206019，H2015423026）