目的 研究黑籽重楼Paris thibetica根状茎的化学成分及其抗肿瘤活性。方法 综合运用正相硅胶、Sephadex LH-20、ODS-C₁₈等柱色谱以及半制备高效液相色谱等方法进行分离纯化，并通过MS、¹H-NMR、¹³C-NMR等波谱学方法进行结构鉴定。采用MTT法研究化合物的体外抗肿瘤活性。结果 从黑籽重楼根状茎的乙醇提取物中分离得到14个化合物，分别鉴定为重楼皂苷V（1）、重楼皂苷I（2）、重楼皂苷II（3）、重楼皂苷VI（4）、偏诺皂苷元-3-O-α-L-呋喃阿拉伯糖基（1→4）-β-D-吡喃葡萄糖苷（5）、重楼皂苷H（6）、偏诺皂苷元-3-O-α-L-吡喃鼠李糖基（1→4）-[α-L-吡喃鼠李糖基（1→2）]-β-D-吡喃葡萄糖苷（7）、重楼皂苷VII（8）、parisyunnanoside G（9）、trikamsteroside E（10）、豆甾醇-3-O-β-D-吡喃葡萄糖（11）、β-谷甾醇棕榈酸酯（12）、β-香树脂醇（13）、4-羟基-5-甲基呋喃-3-羧酸（14）。结论 所有化合物均为首次从黑籽重楼中分离得到，化合物10、12～14为首次从重楼属植物中分离得到。化合物1～8 对人肝癌细胞BEL-7402有细胞毒活性，其中化合物3 活性最好，IC₅₀为0.48 μmol/L。

Objective To investigate the chemical constituents from Paris thibetica and their antitumor activities. Methods The separations and purifications were taken by column chromatography over silica gel, Sephadex LH-20, ODS-C₁₈, and semi-preparative HPLC. The in vitro antitumor activities of the isolated compounds were studied by MTT method. Results Fourteen compounds were isolated from ethanol extract of the rhizomes of P. thibetica, which were identified as paris saponin V (1), paris saponin I (2), paris saponin II (3), paris saponin VI (4), pennogennin-3-O-α-L-arabinofuranosyl-(1→4)-β-D-glucopyranoside (5), paris saponin H (6), pennogennin-3-O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (7), paris saponin VII (8), parisyunnanoside G (9), trikamsteroside E (10), stigmasterol-3-O-β-D-glucopyranoside (11), β-sitosteryl palmitate (12), β-amyrin (13), and 4-hydroxy-5-methylfuran-3-carboxylic acid (14). Conclusion All compounds are firstly reported from P. thibetica, and this is the first report of compounds 10 and 12-14 from the genus Paris L. Compounds 1-8 show cytotoxicities against BEL-7402. Among the tested compounds, compound 3 exhibits the strongest cytotoxicity with IC₅₀ value of 0.48 μmol/L.

国家自然科学基金资助项目（81173487，81373904，81673535）