[关键词]
[摘要]
目的 建立秦七风湿方的HPLC指纹图谱,为其质量评价及药效物质基础研究提供依据。方法 采用Inert Sustain C18色谱柱(150 mm×4.6 mm,5 μm);以0.1%磷酸水溶液-乙腈为流动相进行梯度洗脱;检测波长为203 nm;柱温为30℃;体积流量为1 mL/min;测定10批秦七风湿方样品,利用中药色谱相似度评价系统(2004A版)建立秦七风湿方HPLC指纹图谱,并通过对照品对共有峰进行指认。结果 建立了秦七风湿方HPLC指纹图谱,10批样品的相似度均在0.99以上,共标定19个共有峰,8个峰来源于珠子参,8个峰来源于秦艽,5个峰来源于山茱萸(其中1号峰为珠子参、秦艽和山茱萸共有),并通过与对照品比较指认了其中8个色谱峰,分别为马钱苷酸(2号峰)、莫诺苷(3号峰)、龙胆苦苷(5号峰)、马钱苷(6号峰)、人参皂苷Ro(12号峰)、人参皂苷F1(13号峰)、竹节参皂苷IVa(14号峰)、人参皂苷F2(17号峰)。结论 所建立的方法稳定、准确、重复性好,可为秦七风湿方的质量控制及药效物质基础研究提供依据。
[Key word]
[Abstract]
Objective To establish an HPLC fingerprint of Qinqi Rheumatism Formula (QRF) for its quality control and effective components determination. Methods HPLC method was performed on Inert Sustain C18 (150 mm×4.6 mm, 5 μm) column with gradient elution composed of acetonitrile-aqueous solution containing 0.1% phosphoric acid at the flow rate of 1.0 mL/min. The column temperature was set at 30℃, while the detective wavelength was set at 203 nm. The common mode of HPLC fingerprint for 10 batches of QRF was established with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2004 A edition) and the common peaks were identified by reference compounds. Results Fingerprints of QRF were established. The similarities of the 10 batches of samples were above 0.99. A total of 19 common peaks were found. Eight mutual peaks were from Panacis Majoris Rhizoma, eight mutual peaks were from Gentianae Macrophyllae Radix, and five mutual peaks were from Corni Fructus (Peak 1 was the common peak from Panacis Majoris Rhizoma, Gentianae Macrophyllae Radix, and Corni Fructus). Based on the retention time of reference substances, eight constituents including loganic acid (peak 2), morroniside (peak 3), gentiopicroside (peak 5), loganin (peak 6), ginsenoside Ro (peak 12), ginsenoside F1 (peak 13), panax japonicus IVa (peak 14), and ginsenoside F2 (peak 17) were indentified. Conclusion The method is stable, specific, and reproducible, and can be used for the quality control of QRF and the study of its effective components.
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[基金项目]
国家自然科学基金项目(81373978);陕西省重点科技创新团队(2012KTC-20);陕西省科技资源开放共享平台项目(2015FWPT-01);陕西省教育厅专项科研计划项目(16JK1214)