目的 以白及多糖为主要基质制备水凝胶并进行质量评价。方法 采用水提醇沉法提取白及多糖并用酶解和Sevag法进行纯化。通过傅里叶变换红外光谱、热重分析、热差分析和X射线衍射分析对白及多糖进行表征。将白及多糖作为基质，交联卡波姆940制备得水凝胶。采用旋转流变仪测定水凝胶流变学特性，采用经皮水分散失速率仪测定水凝胶经皮促渗活性，并以止血时间和凝血4项为指标，测定了水凝胶的止血活性。结果 白及多糖水凝胶具有较好的黏弹性和物理强度，且具有经皮促渗活性和止血活性。结论 白及多糖水凝胶在药物透皮传送系统和伤口敷料应用方面具有巨大潜力。

Objective To prepare the hydrogels using Bletilla striata polysaccharides (BSP) as groundmass and evaluate its properties. Methods BSP were successfully extracted and characterized by Fourier Transform Infrared Spectrometer, thermogravimetric analysis, differential thermal analysis, and X-ray diffraction. The BSP was incorporated with Carbopol 940 to prepare hydrogels. Rheological behavior of the gels was investigated by rotational rheometer, skin permeation properties and bioactivities of BSP gels were evaluated by trans-epidermal water loss (TEWL) and blood coagulation examinations respectively using mice as model animals. Results The BSP was pure with complete structure. The BSP gels showed the better viscoelasticity and physical strength from carbopol gel. The gels showed better skin permeation enhancement and hemostatic activity. Conclusion This work demonstrates the skin permeation enhancement and plasma coagulation effects of BSP hydrogels, which show great potential in transdermal drug delivery system and wound dressing.

云南省科技计划项目（2016BAE0024）（21562029）